摘要
目的:建立高效液相色谱法同时测定毛冬青药材中2个三萜皂苷类成分(毛冬青皂苷B1和毛冬青皂苷B2)的含量。方法:采用Phenomenex C18(4.6 mm×150 mm,5μm)色谱柱,以乙腈-0.2%磷酸水溶液为流动相梯度洗脱,流速1.0 mL.min-1,检测波长203 nm。结果:毛冬青皂苷B1和毛冬青皂苷B2线性范围分别为2.57～12.82μg(r=0.9998)和4.51～22.52μg(r=0.9999),平均回收率(n=6)分别为100.6%(RSD=0.94%)和100.7%(RSD=1.5%)。结论:6批样品的测定结果表明,该方法简便、准确,可用于毛冬青药材中毛冬青皂苷B1和毛冬青皂苷B2的含量测定。
Objective：To develop an HPLC method for the quantitative determination of two triterpene saponins（ilexsaponin B1 and ilexsaponin B2） in Ilex pubescens.Methods：The determination was carried out on a Phenomenex C18（4.6 mm×150 mm,5 μm） column.The mobile phase was composed of acetonitrile and water（containing 0.2% phosponic acid） with gradient elution.The flow rate was 1 mL·min-1 and the detection wavelength was 203 nm.Results：The linear ranges of ilexsaponin B1 and ilexsaponin B2 were 2.57-12.82 μg（r=0.9998） and 4.51-22.52 μg（r=0.9999）,respectively;The average recoveries（n=6） of ilexsaponin B1 and ilexsaponin B2 were 100.6%（RSD=0.94%） and 100.7%（RSD=1.5%）,respectively.Conclusion：The determination results of six batch samples show that the method is simple,accurate,and can be used in the quantitative determination of two components（ilexsaponin B1 and ilexsaponin B2） in Ilex pubescens.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第12期2229-2231,共3页
Chinese Journal of Pharmaceutical Analysis
