摘要
目的测定毛冬青药材中毛冬青皂苷B1的含量，首次建立毛冬青药材的专属性含量控制方法。方法采用C18色谱柱；以乙腈-2mL·L^-1 H3PO4（39：61）为流动相，柱温：30℃；流速：0．8mL·min^-1；检测波长：203nm；进样量5μL。结果毛冬青皂苷B1的分离度良好，线性范围为0．5～12．5μg，平均回收率98．24％。结论方法专属性强，重复性好，结果准确，为毛冬青药材的质量控制提供了科学、专属性的质量控制方法。
Objective To establish a method for quality estimation of Radix llicis Pubescentis by RP-HPLC. Methods RP-HPLC method was adopted to determine the content of ilexsaponin B1 by using C18 column. Acetonitril-2 g · L^-1 Ha PO, （39 ： 61） as mobile phase was used to determinate ilexsaponin B1, the column temperature was 30 ℃, the flow rate was 0.8 mL · min^-1 , the UV detection wavelength was set at 203 nm. Results The Ilexsaponin B1 was completely separated from other compositions under established conditions. The liner range of ilexsaponin B1 was 0.5 - 12.5 bag （ r = 0. 999 9, n = 7）, the average recovery of ilexsaponin B1 was 98.24%. Conclusions It was the first time to establish RP-HPLC method with accuracy, good reproducibility for determining the content of ilexsaponin B1 in Radix Ilicis Pubescentis.
出处
《西北药学杂志》
CAS
2008年第4期208-209,共2页
Northwest Pharmaceutical Journal
