摘要
以斑马鱼(Zebra fish)Danio rerio、剑尾鱼Xiphophorus hellerii、孔雀鱼(Guppy)Poecilia reticulata为受试鱼种,采用半静态试验,考察苯、苯酚、氯苯、4-氯苯酚对3种鱼的急性毒性,结果表明,上述4种有机物对斑马鱼的96 h LC50分别为132.22、28.86、9.66、4.29 mg.L-1;对剑尾鱼的96 h LC50分别为123.76、27.25、8.86、4.29 mg.L-1;对孔雀鱼的96 h LC50分别为131.25、27.88、9.33、4.59 mg.L-1,都属于高毒物质。毒性大小为苯<苯酚<氯苯<4-氯苯酚。毒性大小与取代基的类型有关,-Cl的毒性要大于-OH,-Cl和-OH的联合毒性大于单独的-Cl和-OH。孔雀鱼和剑尾鱼对上述有机污染物具有较好的敏感性,结果稳定,重现性好,可以作为急性毒性试验的受试鱼种。
In the research,zebrafish,guppy,and Xiphophorus helleri were used as test fishes to study the acute toxicity of four highly toxic substances benzene,chlorobenzene,phenol,and p-chlorophenol on them through semi static toxicity test.The results indicated that 96 h LC50 of the above chemicals toward zebrafish were 132.22,28.86,9.66,and 4.29 mg/L,toward Xiphophorus helleri were 123.76,27.25,8.86,4.29 mg/L,and toward guppy were 131.25,7.88,9.33,4.59 mg/L respectively.The toxicity sequence for the studied chemicals is benzenephenolchlorobenzenechlorobenzene.The toxicity sequence is relevant to the type of substituting group,the toxicity of –Cl is bigger than –OH,the combined toxicity of –Cl and –OH is bigger than each of them.Both guppy and Xiphophorus helleri were more sensitive to these pollutants,and the result with guppy and Xiphophorus helleri had better reproducibility,so guppy and Xiphophorus helleri are good laboratory fishes for acute toxicity test.
出处
《生态环境学报》
CSCD
北大核心
2011年第11期1720-1724,共5页
Ecology and Environmental Sciences
基金
浙江省科技厅面上农业项目(2009C22G1120001)
关键词
苯
氯苯
n苯酚
4-氯苯酚
急性毒性
剑尾鱼
孔雀鱼
斑马鱼
benzene
chlorobenzene
phenol
chlorobenzene
acute toxicity
guppy
Xiphophorus helleri
zebrafish
