摘要
目的:探讨海藻酸盐支架-骨髓间充质干细胞(BMSCs)复合物在急性肝衰竭大鼠体内应用的可能性,为人工肝组织的体内应用提供基础。方法:BMSCs用氯甲基苯甲酰氨荧光染料(CM-DiI)标记后种植于海藻酸盐支架形成支架细胞复合物。动物分实验组和对照组,构建70%肝切除大鼠模型,实验组大鼠给予支架细胞复合物平铺于肝脏创面上,对照组给予单纯支架。4周后取出支架细胞复合物行荧光显微镜追踪观察;切片行白蛋白及糖原染色追踪支架上细胞的分化;同时比较两组大鼠生存率及肝功能变化情况。结果:CM-DiI能很好地标记细胞。体内应用后BMSCs能够在海藻酸盐支架上分泌白蛋白,合成糖原。实验组大鼠的生存率及肝功能改善情况优于对照组。结论:海藻酸盐支架-BMSCs复合物在急性肝衰竭大鼠体内能够起到部分肝功能支持的作用。
AIM： To evaluate the use of alginate scaffold with bone marrow mesenchymal stem cells（BMSCs） in a rat acute hepatic failure model.METHODS： BMSCs were labeled with chloro-methylbenzamido dialkylcarbocyanine（CM-DiI）,cultured in alginate scaffold and transplanted into the liver of acute hepatic failure rats with 70% liver resection.Four weeks later,the scaffold-cell complexes were taken out,and their abilities to secret albumin（ALB） and store glycogen were examined.The survival rate and liver functions of the rats were also examined.RESULTS： CM-DiI was an effective cell tracer.The BMSCs in alginate scaffold secreted ALB and stored glycogen in acute hepatic failure rats.The survival rate and liver functions of the rats treated with scaffold-cell complexes were better than those of the rats treated with alginate alone.CONCLUSION： Alginate scaffold with BMSCs can be used as artificial hepatic tissues in acute hepatic failure rats.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2011年第12期2382-2386,共5页
Chinese Journal of Pathophysiology
关键词
海藻酸盐支架
骨髓间充质干细胞
急性肝功能衰竭
人工肝
Alginate scaffold
Bone marrow mesenchymal stem cells
Acute hepatic failure
Artificial liver
