摘要
目的:建立固相萃取分离纯化柚皮苷的方法。方法:以强阴离子交换(strong anion exchange,SAX)填料为吸附材料,利用柚皮苷与填料的功能基团之间的静电作用将柚皮苷吸附在填料上,考察洗脱液离子强度、洗脱液体积,洗脱流速对洗脱效果的影响。结果:选用5mL碳酸氢钠溶液(0.1mol/L,pH9.0)为洗脱液,以0.5mL/min的流速进行洗脱,回收率达到91%以上。结论:本方法简单、高效,选择性好,可为柚皮苷样品的纯化提供理论依据。
Purpose： A solid-phase extraction procedure for the separation and purification of naringin（NG） was developed using strong anion exchange（SAX） cartridge.Method： Naringin was adsorbed onto the cartridge by electrostatic interaction between naringin and functional groups.Several variables affecting the elution performance of SAX,such as ionic strength of elution,elution volume and elution rate,were studied.Results： The application of 5 mL of sodium bicarbonate solution（0.1 mol/L,pH = 9.0） to elute SAX cartridge at a flow rate of 0.5 mL/min resulted in a recovery rate of 91%.Conclusions： The established method is simple and efficient.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第22期51-54,共4页
Food Science
基金
福建省科技计划重点项目(2010Y0035)
