摘要
异戊烯焦磷酸异构酶(IPI)是萜类合成途径的关键酶之一。本文在丹参转录组高通量数据分析的基础上,对丹参IPI基因(SmIPI)进行了克隆及序列分析。SmIPI全长1234bp,包含681bp的开放读码框,编码226个氨基酸。生物信息学结构分析表明,SmIPI为亲水性α/β蛋白,包含有IPI结构域,在序列组成、结构及活性位点等方面与其他植物的IPI均具有高度的相似性。实时荧光定量PCR分析结果表明,SmIPI在丹参生长的各个时期和不同组织器官中差异表达,其表达受病原菌和茉莉酸甲酯的诱导。
Isopentenyl diphosphate isomerase （IPI） is a key enzyme in terpenoid biosynthetic pathway. By analyzing transcriptome sequences of Salvia miltiorrhiza, a gene which showed high homology with IPI from other plants was found and named as SmlPI. SmlPI consists of 1 243 nucleotides, including a single 681-bp opening reading frame and encoding a 226 amino-acid peptide. Bioinformatics analysis showed that SmIPI belongs to α/β protein and contains an IPl domain. The core structure, sequence and activity sites of SmIPI are highly conserved as compared with IPI from other plants. Quantitative real-time PCR analysis revealed that the gene expressed in different developmental stages and different organs, and could be induced by methyl jasmonate （MeJA） and fungal elicitor signal.
出处
《植物生理学报》
CAS
CSCD
北大核心
2011年第11期1086-1090,共5页
Plant Physiology Journal
基金
陕西师范大学中央高校基本科研业务费重点项目(GK-200901014)
关键词
丹参
异戊烯焦磷酸异构酶
生物信息学分析
实时荧光定量PCR
表达模式
Salvia miltiorrhiza
isopentenyl diphosphate isomerase
bioinformatics analysis
quantitative realtime PCR
expression pattern
