摘要
背景视觉诱发电位（VEP）是记录大鼠成年后视皮层可塑性变化的主要功能性评价指标，单眼形觉剥夺（MD）模型是研究哺乳动物视皮层可塑性存在与否的经典模型，应用上述技术研究成年大鼠模型眼优势移动情况对研究弱视眼的治疗时机有重要的意义。目的应用图形视觉诱发电位（PVEP）的方法评估各周龄正常大鼠和双眼形觉剥夺成年大鼠视皮层可塑性的变化。方法健康SPF级Long—Evens大鼠按其出生后周龄分为3、4、5、6、7周组，3、4、5、6周组每组9只，7周组36只。各周龄大鼠左眼分别遮盖3、5、7d，分别于上述时间点记录大鼠左眼、右眼PVEPP。波振幅，观察左右眼遮盖前后P100波振幅变化，评价眼优势移动情况，确定大鼠视皮层可塑性结束的周龄。7周龄SPF级Long—Evens大鼠行双眼PVEP检测，利用眼睑缝合法分别行双眼形觉剥夺7、10、14d，然后打开右眼，分别于3、5、7d后再打开左眼，再次行双眼PVEP检测，观察眼优势是否发生移动。结果出生后3周龄大鼠，左眼遮盖后3—7d，左眼P100波振幅逐渐下降，而右眼P100波振幅逐渐升高，与术前比较差异均有统计学意义（P〈0．01）。出生后6周龄大鼠左眼遮盖3d，左眼、右眼P100波振幅与术前相比差异均无统计学意义（P〉0．05）；但左眼遮盖后5d、7d，左眼P100波振幅明显下降，右眼P100波振幅明显升高，差异均有统计学意义（P〈0．01）。出生后7周龄大鼠左眼遮盖后3～7d，左眼、右眼大鼠P100波振幅与遮盖前相比，差异均无统计学意义（P〉0．05）。3、4、5周龄大鼠术前C／I比值分别为2．69~0．45、2．58±0．41和2．62±0．32，左眼遮盖后的对侧眼振幅值／同侧眼振幅值（C／I）比值分别为1．07±0．15、1．16±0．16和1．14±0．15，差异均有统计学意义（P〈0．01），但6周龄、7周龄大鼠C／I比值分别为2．80±0．48和2．59
Background The visual evoked potential （VEP） is an important functional index to assess visual cortex plasticity. Monocular form deprivation （MD） of rat is a classic model to study visual cortex plasticity change. Utilizing VEP technique record the shift of ocular dominance in adult rats model is of an important significance for explore the treating opportunities of amblyopia. Objective The present study was to observe the pattern VEP changes in different ages of Long-Evens rats and the adult rats after binocular form deprivation. Methods Thirty-six healthy SPF Long-Evens rats were divided into PW3,PW4,PW5,PW6 groups according to the postnatal weeks,and PW7 rats included 36 SPF Long-Evens rats. The left eye lids of the rats were sutured in PW3, PW6 and PW7 rats for 1 ,5,7 days respectively,and PVEP in both left and right eye were recorded to assess the rat age that visual cortex plasticity ended. Bilateral PVEP were recorded in PW7 rats, and the bilateral eyelids were sutured to establish the bilateral form deprivation models. The right eyes were opened in 7,10,14 days, and the left eyes were opened in the next 3,5,7 days respectively and the PVEPs were recorded again to find the shift of ocular dominance and whether binocular form deprivation induce the visual cortex plasticity in adult rats. Results The P100 amplitudes of the left eyes were gradually declined and those of the right eyes were raised from 3 to 7 days after MD of the left eyes in rats of PW3 group in comparison with before MD （P〈0.01）. In PW6 groups,no significant changes in P100amplitudes of both the right and left eyes were found in the third day after MD ,but significant raise in the right eyes and lowing in the left eyes were seen in the 5 and 7 days after MD in comparison with before MD（P〈0.01 ）. No any P100changes in both eyes were found in 3-7 days after MD compared with before MD in PW7 rats （P〉0. 05）. The C/I ratio （contralateral VEP amplitude to ipsilateral VEP amplitude in occluded eyes） in the rats of PW3
出处
《中华实验眼科杂志》
CAS
CSCD
北大核心
2011年第12期1106-1110,共5页
Chinese Journal Of Experimental Ophthalmology
基金
国家自然科学基金项目（30801278、30672280）、国家高科技研究发展计划（863计划）项目（2007AA04Z324）
关键词
视觉诱发电位/图形刺激
视皮层
眼优势
形觉剥夺
Visual evoked potential/pattern stimulate
Visual cortex
Ocular dominance
Form deprivation
