摘要
对分离自大连海域的海洋生物共附生真菌进行了抗菌活性的初筛与复筛,发现有6株具有抗表皮葡萄球菌和铜绿假单胞菌的活性,发现培养基成分及培养方式对活性菌株抗菌物质的产生有显著影响。菌株11-N2抗菌活性最强,其发酵液抗表皮葡萄球菌的效价相当于为氨苄青霉素11.4 U/mL,抗铜绿假单胞菌的效价相当于氨苄青霉素6.0 U/mL。通过硅胶柱层析对该菌株提取物的抗菌活性成分进行了初步追踪,发现二氯甲烷∶甲醇=15∶1洗脱组分具有最强活性,薄层层析显示该组分主要成分在254 nm紫外光下具有强吸收,薄层显色反应提示其主要成分可能为含胺基的甾体或三萜类、有机胺类、吲哚衍生物类化合物。
Marine symbiotic fungi isolated from Dalian sea area were prescrecned and re-screened for investigating their antibacterial activity. Six strains with activities against Staphylococcus epidermidis and Pseudomonas aeruginosa were obtained. It was discovered that the constituents of the culture medium and the method of cultivation had great impact on the antibiotics＇ production of the active strains. Among these fungi, the strain 1 l-N2 exhibited the strongest antibacterial activity and its ferment broth ＇ s titers relative to ampicillin were equal to 11.4 U/mL against S. epidermidis and 6.0 U/ mL against P. aeruginosa. By silica gel column chromatography, the antibacterial active ingredients in its extracts were preliminarily traced and the fraction eluted by methylene chloride/methanol （15：1 ） was found to be the most potent. Thin-layer chromatography showed that the main substances in this fraction had a strong absorption of 254 nm UV light. This suggested that the main substances may be steroids or triterpenoids containing amine groups, organic amine, and indole derivatives.
出处
《天然产物研究与开发》
CAS
CSCD
2011年第6期1025-1030,共6页
Natural Product Research and Development
基金
国家自然科学基金项目(20902009)
大连市优秀青年科技人才基金项目(2008J23JH041)
中国博士后科学基金(2011M500051)
大连交通大学大学生科技创新基金(201006)
中国科学院实验海洋生物学重点实验室开放基金(Kf201009)
辽宁省高校优秀人才支持计划(2009R08)
关键词
海洋真菌
抗菌活性
表面葡萄球菌
铜绿假单胞菌
效价测定
marine fungi
antibacterial activity
Staphylococcus epidermidis
Pseudomonas aeruginosa
titer determination
