摘要
采用对二甲氨基苯甲醛分光光度法测定奶粉及乳饮料中L-羟脯氨酸,具有较好的稳定性。奶粉及乳饮料经浓盐酸水解,得到的L-羟脯氨酸经过氯胺T氧化,与对二甲氨基苯甲醛反应生成红色物质,在558nm下测定吸光值。该方法操作简单、准确率高、检测费用相对少,可用于企业奶粉原料掺水解蛋白及产品中是否掺有水解蛋白的检测提供依据。
The method of P-dimethylaminobenzaldehyde spectrophotom etry was used to determine L-hydroxyproline in raw milk powder and milk drink.The result was stable and the recovery rate was high.After hydrolyzed with acid,L-hydroxyproline was released and oxidized by chloramine T.L-hydroxyproline oxide reacted with P-dimethylaminobenzaldehyde and formed red compounds,the absorbance was detected at 580 nm.The method was simple and accurate.Compared with other detecting methods,the expense of it was smaller.It could provide reference for enterprises to detect whether product was adulterated hydrolyzed protein in raw milk powder and milk drink.
出处
《食品科技》
CAS
北大核心
2011年第12期291-294,共4页
Food Science and Technology
关键词
奶粉
乳饮料
掺假
L-羟脯氨酸
分光光度法
milk powder
milk drink
adulteration
L-hydroxyproline
spectrophotometry
