摘要
目的探讨正常成人外周血细胞表面磷脂酰丝氨酸（PS）的表达及其在凝血过程中的作用。方法取10名正常成人外周血（每份5ml），分离血小板、中性粒细胞、淋巴细胞和红细胞，用乳黏素和组织因子（TF）抗体标记流式细胞仪检测PS和TF的表达。通过凝血复钙时间和内源性或外源性凝血因子Xa以及凝血酶的生成实验测定各细胞组分的促凝活性，并用乳黏素和TF抗体封闭PS和TF，观察其促凝血拮抗作用。结果正常成人外周血细胞表面有少量PS的表达，阳性率分别为：血小板9．1％、中性粒细胞5．4％、淋巴细胞3．9％、红细胞3．2％；凝血复钙时间试验示血小板、中性粒细胞、淋巴细胞和红细胞分别缩短凝血复钙时间47％、36．5％、25％和12．5％；内源性或外源性凝血因子Xa以及凝血酶生成实验结果显示四种正常血细胞成份使活化酶产量增加13％-26％。结论正常血细胞表面具有不同程度的PS表达，并在体内凝血过程中起到一定的促凝作用。
Objective To investigate the expression and procoagulant activity of phosphatidylserine （PS） on the normal peripheral blood cells of adults. Methods Normal peripheral blood samples were collected from 10 healthy volunteers （5 ml from each volunteer） , platelets, neutrophils, lymphocytes and erythrocytes were isolated. The expression and procoagulant activity of PS on normal blood cells were identified by flow cytometry, inhibition test with lactadherin as PS probe and coagulation anticoagulant, respectively. Resuits There was PS expression on a few normal blood cells （9.1%, 5.4% , 3.9% and 3.2% in platelets, neutrophils, lymphocytes and erythrocytes, respectively）. The PS on these normal blood cells in vitro showed significant procoagulant activity. The plasma recalcification time was shortened by 47%, 36.5%, 25% and 12.5% by platelets, neutrophils, lymphocytes and erythrocytes, respectively; the formation of factor X a （through both intrinsic and extrinsic pathways） and thrombin was also increased by 13% -26% by platelets, neutrophils, lymphocytes and erythrocytes, respectively. Conclusion The PS on normal blood cells in vivo may play a crucial role in the coagulation cascade.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2011年第12期858-862,共5页
Chinese Journal of Hematology
基金
黑龙江省自然科学基金（D200911）
关键词
磷脂酰丝氨酸
正常血细胞
凝血复钙时间
Phosphatidylserine
Normal blood cells
Plasma recalcification time
