摘要
目的研究1,25-(OH)2D3对1型糖尿病(T1DM)小鼠的作用及可能机制。方法 40 mg/kg STZ腹腔注射C57BL/6小鼠连续5 d建立T1DM模型,5μg/kg 1,25-(OH)2D3隔日腹腔注射,监测血糖、体质量、饮水及饲料消耗,分析各脏器的病理及超微结构。结果 1,25-(OH)2D3治疗14 d后,T1DM小鼠的血糖、饮食、饮水量显著下降(均P<0.05);1,25-(OH)2D3可减轻胰岛炎症状及各脏器的超微结构病变,诱导淋巴细胞的凋亡,并在胰腺中可见典型自噬现象。结论 1,25-(OH)2D3可诱导胰腺细胞的自噬,同时诱导淋巴细胞凋亡,从而减轻T1DM症状。
Objective To study the effects and the underlying mechanism of 1,25-（OH）2D3 in a mouse model of type 1 diabetes melitus（T1DM）.Methods The experimental mice were given intraperitoneal injections of freshly prepared streptozotocin（STZ,40 mg/kg） during 5 consecutive days to induce T1DM model.Mice metabolic parameters including body weight,food and water intake and blood glucose were monitored periodically.Pathological changes of mice pancreas and kidney were observed,and the expressions of VDR were measured by immunohistochemistry assay.The effects of 1,25-（OH）2D3 on autophagy and apoptosis were observed by electron microscopy.Results After treatment with 1,25-（OH） 2D3 for 14 days,food and water intake and random blood glucose were continuously declined（all P0.05）;inflammation in pancreas and kidney was markedly relieved;and the expression of VDR increased;the apoptotic lymphocytes in spleens were incremental,and the ultrastructure abnormality in pancreases and kidneys was improved.Autophagic vacuoles were visible in pancreatic acinar cells and duct cells.Conclusion 1,25-（OH）2D3could induce autophagy in pancreatic cells and promote the apoptosis of lymphocytes to reduce their immune attack on pancreatic islets,then relieve the symptoms of diabetes.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2011年第5期713-717,共5页
Suzhou University Journal of Medical Science
基金
苏州市应用基础研究计划项目(SZP201001)
