摘要
【目的】建立致敏佝偻病动物模型并检测补充维生素D(vitamin D,VD)前后25-(OH)D3、IgE、IFN-γ及IL-4变化,为儿科临床佝偻病及过敏性疾病防治提供实验依据。【方法】选用SD大鼠,建立致敏佝偻病模型,随机分为补D 30万U组(7只)、补D 60万U组(7只)、对照组(6只),实验组采用一次性肌注补D方法,饲养20d天后采用ELLISA方法及流式细胞仪检测大鼠过敏及免疫指标。【结果】补D组较对照组比较,30万U维生素D可使致敏佝偻病SD大鼠外周血过敏指标IgE降低(P<0.01),而60万U维生素D则使IgE上升(P<0.01);30万U组IFN-γ的UR值上升(P<0.05),但仍较正常鼠低,IL-4的UR值有所下降(P<0.05);60万U组IFN-γ的UR值不但没有上升,反而下降更加明显(P<0.05),IL-4的UR值则上升明显(P<0.05)。【结论】一次性肌注30万U维生素D可以改善过敏机体的免疫状态,而当剂量达60万U时则加重过敏机体Th1/Th2细胞失衡,进一步加重机体的过敏反应。
[Objective] To establish an animal model of rickets that sensitized for measuring their concentration include 25-（OH）Da .IgE.IFN-γ.IL-4 in venous blood after supply vitamin D, as a reference in prophylaxis and treatment of rickets and systemic anaphylaxis disease in pediatry. [Methods] The SD rats model of rickets that sensitized were divided into 3 groups in experiment include 3×10^5 U doses of vitamin D group （7 rats）,6×10^5 U doses of vitamin D group （7 rats） .control group（6 rats）, all the way to a muscle column for experimental groups ,the method include of Elisa and flow cytometry used to test allergy and immunity target of rats after raising 20 days. [Results] The experimental groups compared with the control group, 3 ×10^5U doses of vitamin D decreased the allergy target -IgE of the SD rats（P〈0. 01）, but 6×10^5U doses of vitamin D made IgE increased（P〈0.01）, the UR value of IFN-γ of small dose group increased（P〈 0.05）, but still under normal rats, the UR value of IL-4 decreased; the UR value of IFN-γ of 6×10^5 U doses group was not only ascend, but also descend evidently（P〈0.05）, but the UR value of IL-4 increased（P〈0.05）. [Conclusion] 3×10^5 U doses of vitamin D could improve this unbalance , but 6 ×10^5 U doses can intensify Th1/Th2 unbalance of allergy body, further increase the physical allergy .
出处
《中国儿童保健杂志》
CAS
2011年第12期1103-1106,共4页
Chinese Journal of Child Health Care
基金
黑龙江省攻关课题基金(GA07C303109)
