摘要
以油菜蜂花粉为研究对象,根据溶解性的不同,从中分离出了水溶性蛋白、醇溶性蛋白、碱溶性蛋白及盐溶性蛋白,并对各蛋白组分抗氧化活性进行了研究。结果表明:油菜蜂花粉中主要蛋白为水溶性蛋白,占其干重的7.35%,其次为醇溶性蛋白3.65%,盐溶性蛋白和碱溶性蛋白分别占2.50%和2.24%。4种不同溶解性的油菜蜂花粉蛋白均具有一定的体外抗氧化作用,各蛋白组分的总抗氧化能力以及对DPPH自由基、OH.和O2-.的清除能力呈现较好的量效关系;4种蛋白抗氧化活性由强到弱的顺序依次为水溶性蛋白>醇溶性蛋白>碱溶性蛋白>盐溶性蛋白。因此,油菜蜂花粉蛋白质可作为一种潜在的天然抗氧剂进行深入研究。
In this paper, rape bee pollen was used as the research object. Four types of rape bee pollen protein, including water-soluble, ethanol-soluble, salt-soluble and alkali-soluble protein were extracted by their different dis- solving abilities. The antioxidant activities were investigated. The results showed that the contents of water-soluble protein, ethanol-soluble protein, alkali - soluble protein and salt-soluble protein were 7.35 % , 3.65 % , 2.50 % , and 2.24 % , respectively. All four protein components from rape bee pollen had antioxidant activity. The relation- ship between concentration and the total antioxidant power, the scavenging power on DPPH, OHo, and 0-20 all showed positive correlation with the concentration. Antioxidant activity from strong to weak was in the following order： water-soluble protein 〉 ethanol-soluble protein 〉 alkali-soluble protein 〉 salt-soluble protein. Therefore, rape bee pol- len proteins have the potential as natural antioxidant.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2011年第10期218-222,共5页
Food and Fermentation Industries
基金
西北大学校内基金(NG10002)
西北大学科研启动金(PR10037)
