摘要
目的观察不同年龄患者骨髓间充质干细胞(hMSCs)体外增殖特性。方法使用密度梯度离心法分离患者骨髓hMSCs,对其进行培养并保留贴壁细胞传代。观察项目:细胞生长情况,第1、2、3代细胞传代时间,扩增至治疗所需细胞数量时间。20例患者分组:A组(11个月～3岁)6例、B组(4～20岁)6例、C组(21～40岁)5例、D组(41～60岁)3例。结果 (1)原代时间:A、B和C组3组首次传代时间相仿(t=0.12,t=0.55,P>0.05)差异无统计学意义,D组首次传代时间明显长于其他3组,(t=2.73,P<0.05)。(2)第2次传代时间:A组和B组明显短于C组和D组(t=4.84,P<0.01);(3)第3次传代时间:A组最快(t=2.42,P<0.05),其余3组传代时间差异无统计学意义(t=1.23,t=0.64,P>0.05)。(4)20岁以内患者培养达到治疗数量细胞所需的时间明显小于20岁以上年龄的患者(t=4.06,P<0.01),A组以及B组所需时间差异无统计学意义(t=0.97,P>0.05)。结论 hMSC的体外培养增殖速度随着年龄的增加而降低。
Objective To determine whether proliferation and differentiation capability of human mesenchymal stem cells（hMSCs） might be influence by age. Methods hMSCs cultures were obtained by using density centrifuge method from 20 donors aged from 11 months to 56 years old,including 6 cases less than 3 years old as group A,6 cases less than 20 years old as group B,5 cases less than 40 years old as group C and 3 cases less than 60 years old as group D. The cell growth,the time required for passage1,2 and 3 generation and the time required for amplification to needed cell population were investigated. Results There was no significant difference of the first passage time between group A,B and C （t=0.12,t=0.55,P〉0.05） ,but that of group D was longer than the previous 3 groups （t=2.73 ,P〈0.05）. The second passage time of group A and B was significantly shorter than the other two groups （t=4.84,P〈0. 001）. The third passage time of group A was the shortest （t=2.42,P〈0.05） ,but those of the other three groups were not significantly different （t~ 1.23, t~ 0.64, P^0.05）. The time required for amplification to needed cell popu- lation of group A and B was shorter than that of group C and D （t=4.06 ,P^0.01） ,but not significantly different between group A and B （t=0.97 ,P〉0.05）. Conclusion The in vitro proliferation rate of hMSC decreased with the age increasing of donors.
出处
《国际检验医学杂志》
CAS
2011年第19期2189-2191,共3页
International Journal of Laboratory Medicine
基金
国家重点基础研究发展计划(2011CB965101)
关键词
间质干细胞
细胞培养
增殖
年龄
mesenchymal stem cells
cell culture
proliferation
aging
