摘要
目的:研究孕酮预处理对视网膜缺血再灌注损伤后神经元凋亡的影响,并探讨其对视神经保护的可能机制。方法:用升高眼压的方法制作兔视网膜缺血再灌注模型,将36只新西兰大白兔随机分为正常组(n=4)、对照组(n=16)和孕酮组(n=16)。缺血再灌注模型制作前24 h,给予对照组兔耳缘静脉注射生理盐水4 ml/kg,孕酮组兔耳缘静脉注射孕酮4 mg/kg。观察缺血再灌注后不同时段对照组与孕酮组视网膜组织学变化和Bcl-2的蛋白表达及凋亡情况。结果:视网膜缺血再灌注各时间段,HE染色显示,孕酮组视网膜内层厚度均较对照组厚,且内核层细胞数较多,差异均有统计学意义(均P<0.05);凋亡检测结果显示,缺血再灌注24 h时凋亡达高峰,孕酮组内核层的凋亡细胞少于对照组,差异有统计学意义(P<0.05);孕酮组和对照组在缺血再灌注12 h时均检测到Bcl-2蛋白表达,孕酮组Bcl-2蛋白表达水平较对照组少(P<0.05)。结论:孕酮预处理可以促进视网膜缺血再灌注后细胞的存留,减少细胞凋亡,对视神经损伤具有保护作用。
Objective： To study the influence of progesterone pretreatment on neuronal apoptosis after retinal ischemia reperfusion injury and investigate its potential protection mechanism for optic nerves.Methods： The intraocular pressure elevation method was used to build the rabbit retinal ischemia reperfusion model.36 New Zealand white rabbits were randomly divided into normal group（n=4）,control group（n=16） and progesterone group（n=16）.24 h before ischemia,the control group was treated with intravenous injection of normal saline 4 ml/kg in the ear margin,while the progesterone group was treated with 4 mg/kg of progesterone.The retinal histological changes,Bcl-2 protein expression and apoptosis of control group and protestation group in different time periods after ischemia reperfusion were observed.Results： In different time periods after retinal ischemia reperfusion,HE staining showed that the retinal inner layer thickness and the number of inner nuclear layer cells of progesterone group was larger than that of the control group（P0.05）;apoptosis test results showed that the apoptosis reached peak at 24 h after ischemia reperfusion,and the apoptotic cells in the inner nuclear layer of the progesterone group were less than those of the control group（P0.05）;both the progesterone group and the control group showed Bcl-2 protein expression at 12 h after ischemia reperfusion,and the expression of the progesterone group was less than that of the control group（P0.05）.Conclusion： Progesterone pretreatment can promote cell persistence after retinal ischemia reperfusion,reduce cell apoptosis and protect injured optic nerves.
出处
《中国医药导报》
CAS
2011年第35期15-16,19,共3页
China Medical Herald
关键词
孕酮
视网膜缺血
视神经损伤
凋亡
神经保护
Progesterone
Retinal ischemia
Optic nerve injury
Apoptosis
Neuroprotection
