期刊文献+

硫氧还蛋白在糖尿病大鼠视网膜中的动态表达 被引量:2

Dynamic expression of thioredoxin in retina of diabetic rats
下载PDF
导出
摘要 目的观察硫氧还蛋白(thioredoxin,Trx)在糖尿病(diabetes mellitus,DM)大鼠视网膜中的表达部位及动态变化规律。方法成年Sprague Dawley(SD)大鼠随机分为2组,糖尿病组(DM组)一次性腹腔注射链脲佐菌素制作大鼠DM模型;对照组(NC组)注射同等剂量的柠檬酸缓冲液。在注射后4周、8周、12周,采用免疫组织化学法检测Trx在各组大鼠视网膜中的定位表达,采用Real-timePCR测定视网膜Trx mRNA表达。结果免疫组织化学结果显示,NC组与DM组大鼠视网膜神经纤维层、神经节细胞层、内核层有Trx阳性表达,表达位于细胞浆。Real-timePCR结果显示正常大鼠视网膜有Trx mRNA的表达;与NC组相比,DM组各时间点Trx mRNA表达下降,4周时DM组Trx mRNA相对表达量为0.42±0.03,约为NC组(1.00±0.03)的0.42倍;8周时DM组Trx mRNA相对表达量为0.57±0.04,约为NC组(1.00±0.03)的0.57倍;12周时DM组Trx mRNA相对表达量为0.82±0.08,约为NC组(1.00±0.07)的0.82倍,差异均有统计学意义(均为P<0.05)。DM组视网膜组织TrxmR-NA在4~12周表达逐渐升高,至第12周时仍未达到正常水平,第4周与第12周相比差异有统计学意义(P=0.00)。结论在糖尿病视网膜病变时,Trx mRNA表达下降,说明氧化应激反应产生过多的活性氧使得Trx mRNA表达量下降。随着DM病程的延长,视网膜Trx mRNA表达量逐渐升高,这可能是Trx系统功能下降、自由基增多引起的一种代偿性反应。 Objective To observe the position and dynamic changes of thioredoxin(Trx) in the retina of diabetic rats.Methods Adult Sprague Dawley rats were randomly divided into two groups:normal control(NC) group and diabetes mellitus(DM) group.Rats in DM group were injected streptozocin.Rats in NC group were injected equal amount of citric acid buffer.Immunohistochemistry was used to observe the protein expression of Trx at 4 weeks,8 weeks and 12 weeks after injection.The mRNA levels of Trx were determined by real-time PCR.Results Immunohistochemistry was showed that the expression of Trx was in the nerve fiber layer,retinal ganglial cell layer and inner nuclear layer both in NC group and DM group.The results of real-time PCR showed that Trx mRNA was expressed in the retina of normal rats.Compared with those in NC group,the mRNA levels of Trx in DM group decreased.The mRNA relative level of Trx in DM group was 0.42±0.03 at 4 weeks,which was about 0.42 times to that(1.00±0.03) of NC group;The mRNA relative level of Trx in DM group was 0.57±0.04 at 8 weeks,which was about 0.57 times to that(1.00±0.03) of NC group;The mRNA relative level of Trx in DM group was 0.82±0.08 at 12 weeks,which was about 0.82 times to that(1.00±0.07) of NC group,there were statistical differences(all P0.05).The mRNA levels of Trx in DM group increased from 4 weeks to 12 weeks.But at week 12,the mRNA level of Trx did not reach the normal level.There were significant differences between the DM group at 4 weeks and at 12 weeks(P=0.00).Conclusions The expression of Trx mRNA decreases in diabetic retinopathy.It is caused by the excessive ROS which are generated by oxidative stress.With the development of diabetes,the expression of Trx mRNA gradually increases in the retina.That may be a compensatory response to the decreasing of Trx system functions and increasing of free radicals.
出处 《眼科新进展》 CAS 北大核心 2011年第12期1123-1125,1129,共4页 Recent Advances in Ophthalmology
基金 辽宁省教育厅高等学校科研项目计划(编号:20060994)~~
关键词 糖尿病视网膜病变 硫氧还蛋白 氧化应激 diabetic retinopathy thioredoxin oxidative stress
  • 相关文献

参考文献12

  • 1孙文涛,张小玲,高嵩.糖尿病性视网膜病变发生发展的相关因素[J].国际眼科杂志,2005,5(4):755-759. 被引量:43
  • 2Pillay CS, Hofmeyr JH, Rohwer JM. The logic of kinetic regulation in the thioredoxin system[ J]. BMC Syst Biol,2011,5 ( 1 ) : 15. 被引量:1
  • 3Yamawaki H,Haendeler J,Berk BC. Thioredoxin: a key regulator of cardiovascular homeostasis [ J ]. Circ Res, 2003,93 ( 11 ) :1029-1033. 被引量:1
  • 4Arner ES, Holmgren A. The thioredoxin system in cancer [ J ]. Semin Cancer Biol,2006,16 (6) :420-426. 被引量:1
  • 5Matsuo Y, Hirota K, Nakamura H, Yodoi J. Redox regulation by thioredoxin and its related molecules [ J ]. Drug News Pe, rspect , 2002,15 (9) :575-580. 被引量:1
  • 6Brownlee M. The pathobiology of diabetic complications: a unif- ying mechanism [ J ]. Dlabetes, 2005,54 ( 6 ) : 1615 -1625. 被引量:1
  • 7李媛,卢艳.糖尿病视网膜病变早期发病机制研究进展[J].国际眼科杂志,2005,5(4):750-754. 被引量:40
  • 8程丽霞,隋国良,董砚虎.糖尿病视网膜病变的研究进展[J].国外医学(内分泌学分册),1999,19(2):73-75. 被引量:23
  • 9Kasun0 K,Nakamura H,Ono T,Muso E,Yodoi J. Protective roles of thioredoxin, a redox-regulating protein,in renal ischemia/reper- fusion injury[J]. Kidney Int,2003,54(4) :1273-1282. 被引量:1
  • 10Shao LE, Tanaka T, Gribi R,Yu J. Thioredoxin-related regulation of N0/NOS activities[ J ]. Ann N Y Acad Sci,2002,962 : 140-150. 被引量:1

二级参考文献61

共引文献105

同被引文献29

  • 1Moore EC, Reichard P. Enzymatic synthesis of deoxyribonucleotides. VI. The cytidine diphosphate reductase system from novikoff hepatoma [ J ]. J Biol Chem, 1964,239 : 3453-3456. 被引量:1
  • 2Yegorova S,Liu A,Lou MF. Human lens thioredoxin:molecular cloning and functional characterization [ J ]. Invest Ophthalmol Vis Sci, 2003, 44 : 3263-3271. 被引量:1
  • 3Lou MF. Redox regulation in the lens[ J]. Prog Retin Eye Res,2003, 22 : 657-682. 被引量:1
  • 4Burke-Gaffney A, Callister ME, Nakamura H. Thioredoxin : friend or foe in human disease[ J ] ? Trends Pharmaeol Sci,2005,26 : 398-404. 被引量:1
  • 5Powis G, Monffort WR. Properties and biological activities of thioredoxins [ J ]. Annu Rev Pharmacol Toxicol,2001,41: 261-295. 被引量:1
  • 6Xing KY, Lou MF. Effect of age on the thiohransferase (glutaredoxin) and thioredoxin systems in the human lens [ J ]. Invest Ophthalmol Vis Sci ,2010,51 : 6598-6604. 被引量:1
  • 7Fernando MR, Nanri H, Yoshitake S, et al. Thioredoxin regenerates proteins inactivated by oxidative stress in endothelial ceils [ J]. Eur J Biochem, 1992,209 : 917-922. 被引量:1
  • 8Yoshitake S, Nanri H, Fernando MR, et al. Possible differences in the regenerative roles played by thioltransferase and thioredoxin for oxidatively damaged proteins [ J]. J Biochem, 1994,116 : 42-46. 被引量:1
  • 9Speetor A, Yan GZ, Huang RR, et al. The .effect of H202 upon thioredoxin-enriched lens epithelial cells[ Jl. J Biol Chem, 1988,263 : 4984-4990. 被引量:1
  • 10Moon S,Fernando MR,Lou MF. Induction of thioltransferase and thioredoxin/ thioredoxin reductase systems in cultured porcine lenses under oxidative stress[J]. Invest Ophthalmol Vis Sci ,2005,46 : 3783-3789. 被引量:1

引证文献2

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部