摘要
目的:探讨安宫牛黄丸对脑缺血模型的保护作用及血流变的影响。方法:将大鼠随机分为假手术组、模型组(生理盐水12 mL.kg-1ig)、安宫牛黄丸组(0.25 g.kg-1安宫牛黄丸溶解液ig)、西药组(20%甘露醇10 mL.kg-1、速尿1.5 mg.kg-1、胞磷胆碱钠注射液20 mg.kg-1尾静脉注射)、中西药组(西药组+安宫牛黄丸组),采用线栓法制备大脑中动脉梗死(MACO)大鼠模型,连续给药4 d后断头取脑,在脑缺血后第1,2,3天进行卒中指数评分,光镜下观察各组脑缺血4 d后缺血脑组织病理形态学变化,检测血液流变学指标的变化。结果:脑缺血4 d后全血黏度(ηb)、血浆黏度(ηp)、血小板聚集率(PAgT)、红细胞聚集指数(EAI)、红细胞刚性指数(IR)明显升高,红细胞变形指数(DI)明显降低,安宫牛黄丸组、西药组、中西药组均可不同程度地降低脑卒中指数,降低脑缺血大鼠的ηb,ηp,PAgT,EAI,IR并增加DI,以中西药组作用最为显著(P<0.05或P<0.01)。结论:安宫牛黄丸对脑缺血损伤具有脑保护作用,这可能与其降低血液黏度作用有关。
Objective：To investigate the protective effect of Angong Niuhuang Wan on the cerebral ischemia and blood rheology in the rats.Method： Rats were randomly divided into sham group,vehicle group（normal saline was poured into the stomach at 12 mL·kg-1）,Angong Niuhuang Wan group（Angong Niuhuang Wan solution was at 0.25 g·kg-1）,Western medicine group（mannitol at 10 mL·kg-1 furosemide injection at 1.5 mg·kg-1 and citicoline sodium injection at 20 mg·kg-1 were determined tail vein injection）,integrated Chinese and Western medicine group.The model of focal cerebral ischemia of rat was established by the sutureoccluded method.Medications were administered after four days continuously of middle cerebral artery occlusion（MCAO） and stroke index evaluation was respectively studied at the first,second and third day after MCAO.By HE coloring,to observe the ischemic tissue after MCAO in four days later under the light microscope and examine the change of hemorheology.Result：After MCAO in four days the whole blood viscosity（ηb）,plasma viscosity（ηp）,platelet aggregation rate（PAgT） erythrocyte aggregation index（EAI）,erythrocyte rigidity index（IR） in vehicle group were much higher than those in sham group（P0.05） and the DI in vehicle group was apparently lower than that in control group（P0.05）;all the treatment groups could decrease the stroke index,the ηb,ηp,PagT,EAI and IR and increase the deformability index（DI） at different levels.Integrated traditional and Western medicine treatment showed more significant effect compared with other groups（P0.05,P0.01）.Conclusion： Angong Niuhuang Wan may protect brain from ischemia injury.The effect of Angong Niuhuang Wan might be related with improvement in hemorheological parameters.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第23期188-191,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
安宫牛黄丸
大脑中动脉梗死大鼠模型
血流变
Angong Niuhuang Wan
middle cerebral artery occlusion model of rats
hemorheology
