摘要
目的:探讨尿中性粒细胞明胶酶相关脂蛋白(neutrophil gelatinase associated lipocalin,NGAL)和N-乙酰-β-D-氨基葡萄糖苷酶(N-acetyl-β-D-glucosaminidase,NAG)对IgA肾病早期诊断价值。方法:选择IgA肾病患者85例,根据Hass分级Hass I～II(A组)40例、Hass III～V(B组)45例;正常对照组30例。应用ELISA法检测尿液NGAL(uNGAL)和uNAG的浓度,并计算uNGAL/Cr比值和uNAG/Cr比值,进而对uNGAL/Cr和uNAG/Cr进行受试者工作特征(ROC)曲线分析,计算ROC曲线下面积和最佳诊断界值。结果:IgA肾病患者的uNGAL、uNAG、uNGAL/Cr和uNAG/Cr均较对照组有显著差异(P均<0.05);uNGAL/Cr和uNAG/Cr ROC曲线面积分别为0.941和0.846,其最佳诊断界值分别为3.50 g/mmol Cr和1.20 U/mmol Cr,取该界值时诊断IgA肾病的敏感度和特异度分别为91.2%、93.7%和83.3%、84.9%。结论:尿NGAL可作为判断IgA肾病早期肾脏损害的客观指标;uNGAL/Cr较uNAG/Cr对IgA肾病损伤诊断的敏感度和特异度高。
Objective：To explore the value of urine NGAL and N-acetyl-β-D-glucosaminidase in diagnosing early injury of IgA nephropathy.Methods： 85 patients with IgA nephropathy were categorized into two groups（Hass I^II group and Hass III^V group）.30 healthy subjects were recruited as controls.uNGAL and uNAG were detected by commercial available ELISA kit according to the manufacturer′s instructions.And the ratio of uNGAL/Cr and uNAG/Cr were calculated.Receiver operating characteristic（ROC） curve analysis was used to evaluat the two on the sensitivity and specificity of diagnosis on IgA nephropathy.The area under the ROC curve and the best diagnostic cut-off value were calculated.Results： The levels of uNGAL,uNAG,uNGAL/Cr and uNAG/Cr are significantly higher in IgAN patients than in control group.The area under ROC curve of uNGAL/Cr and uNAG/Cr were 0.941 and 0.846 respectively.The best diagnostic cut-off value were 3.50 g /mmol Cr and 1.20 U /mmol Cr.Taking the boundary value to diagnose IgA nephropathy,the sensitivity and specificity for uNGAL/Cr and uNAG/Cr were 91.2%,93.7% and 83.3%,84.9% respectively.Conclusion： uNGAL is an objective indicator of early renal damage in IgA nephropathy.uNGAL/Cr has higher sensitivity and specificity for the diagnosis of IgA nephropathy than uNAG /Cr,and it is expected to be diagnostic indicator for IgA nephropathy.
出处
《中国卫生检验杂志》
CAS
2011年第11期2671-2673,共3页
Chinese Journal of Health Laboratory Technology
