摘要
目的探讨肺癌细胞中膜骨架连接蛋白Ezrin对体外培养的肺癌细胞系的增殖、凋亡、侵袭和转移能力的影响,明确Ezrin在肺癌中的异常表达与临床病理因素之间的关系。方法转染EzrinSiRNA后,应用流式、MTT、Transwell和划痕实验等技术评价肺癌细胞体外侵袭转移和增殖能力的变化。结果转染EzrinSiRNA后,划痕实验、MTT和Transwell等实验结果证明肺癌细胞的侵袭和增殖能力减弱。流式细胞仪技术检测凋亡的结果证明Ezrin沉默后,肺癌细胞凋亡数目增加。结论膜细胞骨架连接蛋白Ezrin可以明显促进肺癌细胞的侵袭转移。
Objective To research the influence of Membrane-cytoskeleton linking protein Ezrin on apoptosis,invasiveness and metastasis of lung cancer cells in vitro and to find out the relationship between the clinicopathological factors and the overexpresion of Ezrin in lung cancer.Methods After Ezrin SiRNA transfection,the invasion,metastasis and proliferation changes of lung cancer cells in vitro were evaluated by Flow cytometry,MTT,Transwell,wound healing assay.Results After Ezrin SiRNA transfection,invasion and proliferation ability of lung cancer cells were weakened by MTT,transwell,wound healing assay,and the percentage of apoptosis lung cancer cells increased by flow cytometry technique compared with control.Conclusion Membrane cytoskeleton linking protein Ezrin can significantly promote the invasion and metastasis of lung cancer cells.
出处
《解剖科学进展》
CAS
2011年第6期575-581,共7页
Progress of Anatomical Sciences
基金
国家自然科学基金资助项目(No.30700806)
