摘要
对无细菌、病毒感染的无菌豚鼠和无菌兔与普通豚鼠和普通兔之间的骨髓嗜多染性红细胞微核率进行实验分析比较。带菌环境对豚鼠和兔的遗传物质的稳定性均有一定影响,普通豚鼠和普通兔骨髓嗜多染性红细胞微核率分别比无菌豚鼠和无菌兔高11倍和8倍多。在遗传背景、环境理化因子作用相同情况下,微生物可能是引起微核率升高的直接原因。无菌豚鼠和无菌兔自发微核率低,可作为检测环境中致癌、致畸和致突变物的优选实验动物。
Micronuclei of bone marrow polychromatic erythrocytes of 10 germfree guinea pigs and 4 GF-rabbits were compared respectively with that of 10 conventional guinea pigs and 4 CV-rabbits. It was found that the frequencies of micronuclei in germfree animals were rather lower than that in conventional ones. The frequency of micronuclei in GF guinea pigs was 0.68‰, sharp in contrast to that of 7.55‰. in CV guinea pigs, similarly, 0.72‰ in GF rabbits and 5.7‰ in CV rabbits. The differences between them were statistically significant (P<0.001). We have known that germfree animal can alive much longer than conventional animal though the later were raised in most ideal environment. This is accord with that the genetic material of germfree animal is more steadiness than that of conventional one. In this consequence, we suggested that under the same conditions of genetic backgrounds, physical and chemical factors, the effect may be induced by microorganism-biofactors. But how these biofactors act on bone marrow PCE cells, and how strong is the intensity of the effect on.bone marrow PCE cells? All those still needs further study.
出处
《实验动物与比较医学》
CAS
1990年第3期137-139,共3页
Laboratory Animal and Comparative Medicine
关键词
无菌豚鼠
无菌兔
骨髓嗜多染红细胞
微核率
Micronuclei frequencies
Germfree Guinea pigs
Germfree Rabbits:Bone Marrow Polychromatic crythrocytes
