摘要
目的测定SPF级Hartley豚鼠核心群的生长体质量,血液生理、生化指标,并与清洁级豚鼠各指标进行比较。方法选择种群中雌、雄个体各10只,通过称重,记录0~8周龄体质量;豚鼠采血,应用全自动血细胞计数仪与全自动血液生化分析仪检测各项生理、生化指标。结果在SPF级豚鼠血液生理指标中,白细胞计数(WBC)、中性粒细胞计数(NEUT)、嗜酸性粒细胞计数(EOS)3项指标,在雌性与雄性间有显著性差异(P<0.05);血小板压积(PCT)、血小板容积(MPV)、淋巴细胞计数(LYM)、淋巴细胞百分比(LYM%)、中性粒细胞百分比(NEUT%)、嗜酸性粒细胞百分比(EOS%)、嗜碱性粒细胞百分比(BAS%)在雌、雄性别间表现为差异极显著(P<0.01)。血液生化指标中,碱性磷酸酶(ALP)两性别间有显著性差异(P<0.05),尿素氮(BUN)、肌酐(CREA)、胆固醇(CHO)3项指标,两性别间差异极显著(P<0.01)。与清洁级豚鼠比较结果显示,各项血液学指标中除白细胞计数(WBC)以及雌性豚鼠的红细胞体积(MCV)与血小板计数(PLT)无显著差异(P>0.05)外,其他指标均存在极显著差异(P<0.01)。结论绘制了SPF级豚鼠生长曲线。SPF级豚鼠血液学指标在性别间有差异,与清洁级豚鼠相比存在显著差异。
Objective Measured the growth weight and blood physiological&blood biochemical parameters in SPF Hartley guinea pig core population.Compare with the hematological parameters of clean guinea pig.Method 10 guinea pigs of each sex in the population were selected,and the body weight of 0~8 weeks old was recorded by weighing.Guinea pigs were collected blood,and all physiological and biochemical indexes were detected by automatic blood cell counting instrument and automatic blood biochemical analyzer.Results The WBC、NEUT、EOS have significant difference(P<0.05),the PCT、MPV、LYM、LYM%、NEUT%、EOS%、BAS%have very significant difference(P<0.01)between SPF male and female in blood physiological parameters.The ALP has significant difference(P<0.05),the 3 indexes as BUN、CREA、CHO have very significant difference(P<0.01)between SPF male and female in blood biochemical parameters.The results of comparison with CL guinea pigs show that WBC between SPF and CL of both sex,MCV、PLT between SPF and CL of female have no significant difference(P>0.05),other indexes have very significant difference(P<0.01)between SPF and CL guinea pigs.Conclusion The growth curve of SPF guinea pig was plotted.The hematological parameters of SPF guinea pigs are significantly different from sex and have very significant difference between SPF and clean guinea pigs.
作者
武卫国
范涛
王洪
王学文
刘佐民
柳全明
WU Weiguo;FAN Tao;WANG Hong;WANG Xuewen;LIU Zuomin;LIU Quanming(National Institutes for Food and Drug Control,Beijing 102629,China)
出处
《实验动物科学》
2019年第6期27-32,共6页
Laboratory Animal Science
基金
中国食品药品检定研究院中青年发展研究基金(No.2017C4)
关键词
豚鼠
SPF级
生长曲线
血液生理
血液生化
Guinea pig
SPF
growth curve
blood physiological parameters
blood biochemical parameters
