摘要
采用血小板聚集(PAG)试验比浊法观察发现穿心莲(APN)提取物 F_(01341)在一定剂量范围内呈剂量依赖性抑制二磷酸腺苷(ADP)和血小板活化因子(PAF)诱导的人富血小板血浆(PRP)的 PAG。其对 ADP 诱导第1min 和第5min PAG 的 IC_(50)分别为2.6mg/L和1.4mg/L;对由 PAF 诱导 PAG 的 IC_(50)分别为0.58mg/L 和0.38mg/L。血小板鲁米诺化学发光技术证明 APN F_(01341)2mg/L 即显著抑制静息及刺激后人洗涤血小板的化学发光。
Using a turbidimetric method,the authors found that Andrographis paniculate Nees extract F01341(APNF01341) dose-depandently inhibited platelet aggregation(PAG)induced by adenosine diphosphate(ADP)and platelet activating factor(PAF)in human platelet-rieh plasma(PRP).The IC50 values determined with a range of 0.6～4.8mg/L were,2. 6mg/L and 1.4mg/L for the first and second phase of PAG induced by ADP respectively.The IC50 values with dose range 0.3～1.2mg/L were 0.58mg/L and 0.38mg/L for the first and second phase of PAG induced by PAF respective- ly.APNF01341(2mg/L)also inhibited both resting and PMA or thrombin induced luminol-dependent chemiluminescence of human platelet in washed suspension.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
1996年第S1期125-127,289,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
卫生部资助课题
关键词
穿心莲
血小板聚集
化学发光
活性氧
二磷酸腺苷
血小板活化因子
Andrographis paniculate
platelet aggregation
chemiluminescence
reactive oxygen species
adenosine diphosphate
platelet activating factor
