摘要
目的建立液相色谱-化学发光联用技术测定丹参制剂中四种水溶性成分（丹参素、原儿茶醛、丹酚酸B和丹酚酸A）含量的新方法。方法基于丹参四种水溶性成分对HAuCl4-luminol-H2O2化学发光的增强作用,用Shim pack ODS色谱柱（250mm×4.6 mm,I.D.5μm）,以0.1%磷酸水溶液（v/v）和0.1%磷酸甲醇溶液（v/v）为流动相进行液相色谱梯度洗脱,与化学发光在线检测联用,在26 min内实现了四种丹参水溶性成分的高选择性和高灵敏分析。结果四种丹参水溶性成分的检测限在0.1～8.0μg.L-1的范围内,精密度（RSD）均小于2.5%,测定样品的回收率在95.3%到103.9%的范围内。结论本法选择性好、灵敏度高,重复性好,适用丹参制剂中水溶性成分的含量测定。
Aim To develop a novel method using high-performance liquid chromatography coupled with chemiluminescence detection for the simultaneous determination of four water-soluble components（danshensu,protocatechuic aldehyde,salvianolic acid B,and salvi-anolic acid A） in Salvia miltiorrhiza preparations.Methods Good separations of four water-soluble components were achieved on Shim-pack ODS column（250 mm × 4.6 mm,I.D.5 μm） with a gradient elution using the mixture of aqueous phosphoric acid（0.1%,v/v） and methanol phosphoric acid（0.1%,v/v） within 26 min.The detection limits（S/N = 3） for four water-soluble components were in the range from 0.1 to 8.0 μg·L^-1,respectively.Results The relative standard deviations（RSDs） of precision were below 2.5%.The mean recoveries ranged from 95.3% to 103.9%.Conclusion The proposed method is highly selective and sensitive,reproduci-ble,and suitable for the quality control of four water-soluble components in salvia miltiorrhiza preparations.
出处
《安徽医药》
CAS
2010年第10期1144-1147,共4页
Anhui Medical and Pharmaceutical Journal
基金
安徽省自然科学基金项目(No070413113)
安徽医科大学博士科研资助经费项目(NoXJ2005008)
关键词
液相色谱
化学发光
丹参
水溶性成分
鲁米诺
high-performance liquid chromatography
chemiluminescence
salvia miltiorrhiza
water-soluble component
luminol
