摘要
应用免疫组织化学S-P方法,研究115例胃癌手术标本中nm23基因产物/NDPK的表达及其预后意义。结果显示:1.胃癌原发灶中NDPK表达阳性率59.1％(68/115),淋巴结转移灶中阳性率41.9％(36/86);2.无伴胃周区域淋巴结转移癌者,原发灶中NDPK阳性率86.2％(25/29),明显高于伴有胃周淋巴结转移者(50.0％,43/86),P<0.005;随着胃癌浸润胃壁深度和临床p-TNM分期的进展,原发灶中NDPK阳性率呈递减趋势(分别为P<0.001和P<0.001);3.肿瘤最大径≤3cm者,原发灶中NDPK阳性率84.6％(22/26),>3cm者,阳性率51.7％(46/89),二者差异有显著性(P<0.01);4.随着胃癌原发灶中NDPK表达程度增高,患者术后生存期呈逐渐延长的趋势(P<0.001)。以上结果说明,nm23基因在胃癌的生长、浸润和淋巴结转移过程中起某种抑制的作用;胃癌原发灶中nm23基因产物/NDPK表达阳性者,具有较好的预后。
The expression of nm23 gene product/NDPK was studied in curatively resected human gastric carcinoma, using anti-NDPK polyclonal antibody. Of 115 patients tested, 59.1%(68/115) showed positive staining in the primary lesions, and 41.9%(36/86) were positive in the metastatic regional lymph nodes. A significantly higher positive staining rate was found in patients without regional nodal metastasis (86.2%, 25/29) than in those with nodal involvement (50.0%, 43/86; P<0.005). The NDPK expression in the primary site was inversely related to the depth of tumor infiltration and p-TNM stage (P<0.001 and P<0.001, respectively). Tumors smaller than 3cm showed higher incidence of positive staining (84.6%, 22/26) than others (51.7%, 46/89; P<0.01). A positive relation was observed between NDPK expression in primary lesions and post-operative survival period (P<0.001). Therefore, our data indicate that nm23 gene plays an important suppressor role in the process of tumor growth, infiltration and lymph node metastasis in human gastric carcinoma.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1996年第S1期61-64,共4页
Chinese Journal of Digestion
关键词
基因
抑制
胃肿瘤
二磷酸核苷激酶
Gene,inhibition Stomach neoplasm Nucleoside diphophate kinase
