摘要
目的克隆人Fas基因。方法采用RT-PCR技术从EB病毒转化的人B淋巴细胞株中扩增包含全长阅读框架的FascDNA，与pGEM-T质粒连接，经测序验证。结果RT-PCR扩增Fas的PCR产物经限制性内切酶后生成的片段符合预期大小。氨节育抗性的克隆经测序证实得到的正常人FSS与首次文献报道Fas基因有2个碱基的差异。结论本研究用RT-PCR方法克隆了正常人Fas全长cDNA，为进一步研究Fas的功能奠定了基础。
Objective Normal Fas gene must be taken as control when polymorphism ofFas gene of SLE patients is detected by SSCP method. This research is to clone Fas cDNA.Methods By using the reversed transcription - PCR technique, a full length Fas cDNA 1086bp was.cloned from a B lymphocyte line of the normal human being transformed by EB virus.The fragment was ligated with the pGEM - T and sequenced. Res'Its RT- PCR products.were digested with the restriction enzyme and the size of the produced fragment was inaccord with the expected size. The sequencing result showed that we had cloned full readingframe Fas cDNA which had 2 bases different from the first reported. ConcI'sio' Thisstudy had cloned full reading frame Fas cDNA of the normal human being, providing a basisfor further study on the function of Fas and wild control for SSCP study on Fas gene of SLEpatients.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
1999年第S1期4-6,共3页
Journal of Shanghai Jiao tong University:Medical Science
基金
上海市青年科技启明星计划!96QB14020
国家自然科学基金!39470668
国家自然科学重点资助!3973040
