摘要
以多组分山苍子[Litsea cubeba(Lour)Per]香精油作为复合中药模型。以该香精油中主要抗菌成分柠檬醛为中药靶部位,以能分泌致癌毒素的黄曲霉单细胞作为药物作用对象,吸收当今医学影像领域先进的科学技术,采用多学科交叉策略,将多维显微、瑞利光散射(Rayleigh scattering)、电镜与生化分析4项技术构筑平台, 从细胞、亚细胞和生物大分子三个水平,研究柠檬醛作用于黄曲霉的动静态过程,阐明模拟的中药方剂靶部位对细胞整体的作用规律.发现该醛不仅能改变黄曲霉细胞膜的形态结构、物理学特性及其生物学功能(如对物质吸收的选择通透性,细胞体积调节机制等),而且使细胞膜产生脂质过氧化损伤;进入细胞后,既作用于细胞器(如线粒体、细胞核等),使其产生损伤及区域性分布;又通过干扰细胞内大分子拥挤状态,导致细胞内生物大分子构象的改变、高含量类大分子缔合反应不可逆增强以及因生化反应区域效应丧失而产生的新陈代谢紊乱,揭示该醛能使黄曲霉孢子失去萌发力、菌丝体生长被抑制及产生孢子的能力,在于黄曲霉细胞膜、细胞器及大分子失去了正常结构、功能及相关的调节机制.在实现对柠檬醛抗黄曲霉机理阐明的过程中,在研究思路和方法上进行全新的探索.
Take multicomponent Litsea cubeba(Lour) Per' s essential oil as compound model of traditional Chinese medicine, made citral the major antibacterial component of the essential oil as 'target part', and let single cell of Aspergillus flavus secreting aflatoxin causing cancer as the object of drug, advanced technologies are adopted from medical image area, and the strategies that many subjects were combined. To research static and dynamic process of citral on A.flavus and investigate how the imitated target part of prescription influenced on cell, multi-dimensional microscopy, and Rayleigh light scattering and biochemical analysis technology were used to succeed in it at three levels of cell, sub-cell and molecule. It is not only found that citral damaged the morphological structure of cell membrane of A. flavus and changed its physical property after it invaded the cell, but also citral made cell membrane lipid catalase damage. Citral' not only damaged organelles (for instance mitochondrion and nucleolus), but also changed the status of intracellular macromolecular crowding. These changes resulted in the directional local concentration of organelles, the conformation change of macromolecules, the intensification of the irreversible aggregation reaction of macromolecules with the highest content in protoplasm (i. e. proteins) and the metabolic disorder caused by the lost of regionality of biochemical reactions. So, cells, organelles and macromolecules lost their normal structures and functions. Consequently, the spores of Aspergillus flavus lost their germination ability; and its mycelia lost their ability to produce conidia.
出处
《生命科学研究》
CAS
CSCD
2005年第S1期81-94,共14页
Life Science Research
基金
农业部"十五"科技成果转化项目(2004514)
关键词
山苍子油
柠檬醛
黄曲霉
作用机理
essential oil of Litsea cubeba (Lour) Per
citral
Asperglllus flavus
mechanism
