摘要
建立了LC-MS/MS法测定人血浆中的扎托布洛芬。以瑞舒伐他汀钙为内标,采用C_(18)色谱柱,以甲醇-5mmol/L乙酸铵溶液(70:30)为流动相,采用电喷雾电离源、选择反应监测(SRM)负离子扫描方式检测。扎托布洛芬在0 02～8μg/ml浓度范围内线性关系良好,日内和日间RSD均小于15%,回收率为77.5%～87.6%。24名健康志愿者单剂量口服扎托布洛芬80mg后的主药动学参数为:c_(max)(4.16±1.46)μg/ml,t_(max)(1.54±1.00)h,t_(1/2)(3.89±1.69)h,AUC_(0-∞)(15.20±3.01)μg·h·ml^(-1)。
An LC-MS/MS method was established for the determination of zaltoprofen in human plasma. A C18 column was used with the mobile phase of methanol-5 mmol/L ammonium acetate solution （70 ： 30） and rosuvastatin calcium as internal standard. A tandem mass spectrometer equipped with electrospray ionization source was used as the detector and operated in the negative ion mode. The calibration curve was linear in the concentration range of 0.02 - 8 μg/ml. The inter- and intra-day RSDs were less than 15 % with extraction recoveries of 77.5 % - 87.6 %. A single oral dose of 80 mg zaltoprofen was administered to 24 healthy volunteers. The main pharmacokinetic parameters were as follows： cmax （4.16±1.46）μg/ml, tmax （1.54＋1.00） h, 4/2 （3.89±1.69） h, AUC0-∞ （15.20±3.01） μg·h·ml-1.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2011年第8期618-621,共4页
Chinese Journal of Pharmaceuticals
