摘要
目的探讨大鼠急性肠梗阻不同时段血浆内毒素和D-乳酸的变化。方法 Wistar大鼠随机分为正常对照组(n=8)、假手术组(n=32)和肠梗阻组(n=32),分别于术后8、24、72、168h取下腔静脉血,采用偶氮显色法测定血浆内毒素水平,改良分光光度法测定血浆D-乳酸水平。结果肠梗阻组术后8h血浆内毒素水平明显升高,24h有所下降,后又逐渐升高,至术后168h达高峰,各时点比较,差异有显著性(F=52.75,q=2.67～9.64,P<0.05);肠梗阻组各时点血浆内毒素水平均高于假手术组和对照组,差异有显著性(t=3.24～13.36,P<0.05)。肠梗阻组术后8h血浆D-乳酸浓度升高,呈双峰型变化,至术后168h仍明显高于对照组,差异有显著意义(F=106.28,q=5.36～13.17,P<0.05);肠梗阻组各时点血浆D-乳酸水平均高于假手术组和对照组(t=2.57～8.35,P<0.05);肠梗阻组血浆内毒素与D-乳酸水平呈正相关(r=0.632,P<0.05)。结论血浆D-乳酸水平可作为内毒素血症的预警指标,血浆内毒素水平可作为评价肠道屏障功能损伤程度的指标。
Objective To explore the changes the levels of plasma endotoxin and D-lactate in rats with acute bowel obstruction（ABO） at different time points.Methods Wistar rats were randomly divided into normal control group（8 rats）,sham-operation（SO） group（32 rats）,and ABO group（32 rats）.Blood samples were taken from inferior vena cava at 8,24,72 and 168 hours after operation.Plasma endotoxin level was determined with chromogenic method and plasma D-lactate level by mo-dified spectrophotometry.Results A significant elevation of plasma endotoxin level was found in ABO group 8 hours after operation,and there was a somewhat drop at 24 hours,and it was then raised gradually and reached the peak at 168 hours,the differences between each time point were significant（F=52.75,q=2.67-9.64,P0.05）.The plasma endotoxin level of ABO at different time points was higher than that of SO and control groups（t=3.24-13.36,P0.05）.In ABO group,plasma D-lactate level elevated at 8 hours after surgery,manifesting bimodal pattern,and remained higher than that of the control at 168 hours after surgery（F=106.28,q=5.36-13.17,P0.05）.The D-lactate levels of ABO at different time points were significantly higher than that of the SO and the control（t=2.57-8.35,P0.05）.Plasma endotoxin level was positively correlated with plasma D-lactate level（r=0.632,P0.05）.Conclusion Plasma D-lactate level can be used as an early-warning index for endotoxemia,and endotoxin level can be applied for evaluating the degree of injury of barrier function of intestinal tract.
出处
《青岛大学医学院学报》
CAS
2011年第6期501-503,506,共4页
Acta Academiae Medicinae Qingdao Universitatis
关键词
肠梗阻
内毒素
D-乳酸
大鼠
intestinal obstruction
endotoxin
D-lactate
rats
