摘要
以脂肪酶Lipozyme TL IM为催化剂,研究了合成富含n-3多不饱和脂肪酸磷脂的最佳工艺条件,即以正庚烷:异辛烷:正已烷=8:1:1为超声介质,溶剂(mL)/底物(g)=2,酶用量为25%,超声介质中水分质量分数为2%,超声功率70 W,55℃下连续超声5.5 h,在此条件下酯交换到磷脂上的DHA和EPA总质量分数为22.99%。由此可见,通过超声辅助及酯交换条件的优化,不仅可以大大缩短反应所需的时间,而且也能充分激活Lipozyme TL IM的酶活力,从而提高酯化反应的效率。
The optimum reaction conditions of synthesized phospholipids enriched with n-3 polyunsaturated fatty acids by Lipase（Lipozyme TL IM） were studied.Results showed that： mixed solvent proportion（n-heptane： iso-octane： hexane） was 8：1：1（V/V/V）,solvent and substrate ratio was 2：1,Lipozyme TL IM addition was 25%,water content of organic solvent was 2%,power of Ultrasonic Irradiation reaction was 70 W,temperature was 55 ℃ and reaction time was 5.5 h.Under the optimum conditions of transesterification,the incorporation of EPA and DHA was 22.99%.This showed that the reaction time was reduced and the activity of Lipozyme TL IM was enhanced greatly by assistance of ultrasonic irradiation and optimum conditions of transesterification.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2011年第11期42-46,共5页
Journal of the Chinese Cereals and Oils Association
关键词
超声辅助
脂肪酶
N-3多不饱和脂肪酸
assistance by ultrasonic irradiation
lipase
n-3 polyunsaturated fatty acids
