摘要
目的 检测胃肠道肿瘤患者门静脉血中CEA mRNA和CK-20 mRNA的表达.方法 采用荧光定量逆转录聚合酶链反应（FQ-RT-PCR）方法检测胃肠道肿瘤患者手术前后门静脉血中循环肿瘤细胞标志物CEAm RNA和CK20 mRNA的表达情况,并对上述指标在外周血中的含量进行检测;同时以非肿瘤手术患者作为对照组.结果 胃肠道肿瘤患者手术后门静脉血CK20 mRNA （79.7%）和CEA mRNA（84.4%）的阳性表达率与手术前（62.5%,67.2%）比较差异具有统计学意义（P〈0.05）;手术前后门静脉血CK20 mRNA和CEA mRNA的阳性表达率与外周血（43.8%,51.6%）比较差异均具有统计学意义（P〈0.05）.胃肠道肿瘤患者术后门静脉血中CK20 mRNA（mean=3.00）和CEA mRNA（mean=3.56）表达量与手术前（mean=2.34,2.58）和外周血（mean=1.16,1.93）比较差异均具有统计学意义（P〈0.05）.结论 门静脉血中CK-20 mRNA,CEA mRNA的表达明显高于外周血.
Objective To detect portal venous blood CK20 mRNA and CEA mRNA expression of patients with gastrointestinal cancer. Methods Using fluorescent quantitative reverse transcription polymerase chain reaction （FQ-RT-PCR） method to detection the tumor cells circulating markers CK20 mRNA and CEA mRNA expression in peripheral blood ,preoperative and post-operative portal venous blood with gastrointestinal cancer patients and non-tumor operation patients as a control group. Results The CK20 mRNA and CEA mRNA expression positive rates of post-operative portal venous blood with gastrointestinal cancer patients were 79.7% and 84.4% ;and higher than pre-Operative （62.5%, 67.2% ）（P〈 0. 05）. Both pre-operative and post-operative were significantly higher than peripheral blood （43.8%,51.6%） （P〈0.05）. Experimental group and control group pre-operative and post-operatlve in portal vein and peripheral blood CK20 mRNA and CEA mRNA expression positive rate were significantly different （P^0. 001）. The CK20 mRNA and CEA mRNA ex- pression values of post-operative portal venous blood with gastrointestinal cancer patients were significantly higher than both pre-operative portal vein and peripheral blood （P〈0.05）. Conclusion CK20 mRNA and CEA mRNA were a high level in portal venous blood with gastrointestinal cancer patients more than peripheral blood.
出处
《现代检验医学杂志》
CAS
2011年第5期78-80,共3页
Journal of Modern Laboratory Medicine
基金
温州市科技局资助项目（项目编号：Y20070032）.
