摘要
目的研究CK20mRNA、CD44v6、血管内皮生长因子(VEGF)与结直肠癌肝转移的关系,探讨临床预测结直肠癌早期肝转移的有效的客观指标。方法应用荧光定量逆转录聚合酶链反应(RT-PCR)法检测50例结直肠癌患者回流门静脉血中CK20mRNA,并同时应用免疫组织化学方法测定结直肠癌癌组织中CD44v6、VEGF的表达;并与良性病变对照组10例和正常组织对照组10例比较。结果结直肠癌患者门静脉血CK20mRNA;癌组织中CD44v6、VEGF表达阳性率明显高于良性病变对照组(P<0·01)和正常对照组(P<0·01);结直肠癌组织中CD44v6及VEGF的表达与门静脉血中CK20mRNA的表达有显著相关性(r1=0·933,r2=0·906,P<0·05);同时肝转移组CK20mRNA、CD44v6、VEGF阳性表达率均高于非肝转移组(P<0·05)。结论联合检测CD44v6、VEGF及CK20mRNA预测结直肠癌肝转移,可提高预测的灵敏度及特异性,对于结直肠癌早期肝转移监测具有较高的临床价值。
Objective To obtain some effective objective markers used to predict the early liver metastasis of colorectal tumor, the relationship of liver metastasis of colorectal tumor with associate detection three markers such as CK20 mRNA, CD44v6 and VEGF was studied. Methods The expression of CK20 mRNA in patrol venous blood from 30 colorectal cancer patients was detected by fluorescent quarto RTPCR, and the results of CD44v6 and VEGF in colorectal cancer tissue were determined by means of immunohistochemistry, and then compared with those in control groups. Results The rate of positive expression of CK20 mRNA in colorectal cancer patients＇ patrol venous blood was obviously superior to the level of benign pathological changes controls（ P 〈 0. 01 ）, and significantly higher than that of normal controls （ P 〈 0. 01 ）. The rate of positive expression of CD44v6 and VEGF in colorectal tumor tissue was distinctly superior to the level of benign pathological controls, and remarkable higher than that of normal controls（P 〈 0.01 ）. The positive expression of liver metastasis was also clearly higher than that of no liver metastasis（ P 〈 0. 05 ）. The rate of positive expression of CK20 mRNA in patrol venous blood was evidently correlated to the expression of CD44v6 and VEGF in tumor tissue （ r= 0. 933, r2 = 0. 906, P 〈 0. 05 ）. The results of associate detection of CK20 mRNA, CD44v6 and VEGF were closely related to the incidence of liver metastasis. Conclusions If combined detecting these markers of CK20 mRNA, CD44v6 and VEGF to forecast liver metastasis of colorectal tumor, the sensitivity and specialty of prediction will be improved, there were highly clinical values in predicting in early diagnosis liver metastasis of colorectal tumor.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2006年第21期1486-1489,共4页
Chinese Journal of Surgery
关键词
结肠直肠肿瘤
肿瘤转移
血管内皮生长因子
Colorectal neoplasms
Neoplasm metastasis
Vascular endothelial growth factor
