摘要
目的:观察慢性移植肾肾病(CAN)大鼠肾组织中肝细胞生长因子(HGF)、骨形态发生蛋白(BMP7)的表达及大黄酸对其影响。方法:以F344及Lewis大鼠分别作为供受体,建立CAN大鼠动物模型。将移植后30只大鼠分为治疗组(n=16)及模型组(n=14),另以5只Lewis正常大鼠作为对照组。治疗组予以大黄酸100mg.kg-1.d-1灌胃,模型组及对照组给予等量的0.5%羧甲基纤维素钠溶液。于移植后1个月、2个月及4个月分别收集大鼠的血和尿样,检测肾功能及尿蛋白含量。并于移植后2个月及4个月分别宰杀大鼠,观察各组大鼠移植肾组织病理学变化。并应用免疫组化以及实时荧光定量PCR方法分别检测CAN大鼠肾组织HGF、BMP7以及转化生长因子β1(TGF-β1)的蛋白质表达水平以及mRNA表达水平。结果:(1)与模型组比较,大黄酸治疗组大鼠肾功能改善(P<0.05);肾组织病理检查示肾间质纤维化及间质炎症明显改善(P<0.05);(2)与模型组比较,大黄酸治疗组大鼠肾组织TGF-β1无明显差异,但抗纤维化因子BMP7及HGF明显升高(P<0.05)。结论:(1)大黄酸能改善CAN大鼠肾功能及肾脏慢性病变;(2)大黄酸有可能通过诱导肾组织抗纤维化因子HGF、BMP7表达,抑制肾组织炎细胞浸润,发挥抗肾组织纤维化的作用,从而延缓CAN的进展。
AIM：To investigate the expression of HGF and BMP7 in renal tissue of rats with chronical allograft nephropathy（CAN） and the influence of rhein intervention on their expression. METHODS：We used Fisher rats as donors and Lewis rats as recipients to establish the model of chronic allograft nephropathy.30 rats with transplanting kidneys were randomly divided into two groups：16 rats in blank group and 14 rats in rhein intervention group.5 normal lewis rats were as controls.The intervention group was given rhein oral solution 100 mg·kg-1·d-1 by gavage after transplantation.The blank group and control group were given 0.5% solution of sodium carboxymethyl cellulose.The blood and urine sample were collected at 4 weeks,8 weeks and 16 weeks after transplantation respectively and renal function and total urine protein were examined.Half of rats in each group were killed at 8 weeks and 16 weeks for examining the renal pathology.And then we used immunohistochemical methods and real-time quantitative PCR to detect the protein expression levels and mRNA expression levels of HGF,BMP7 and transforming growth factor β1（TGF-β1） in CAN rats kidney tissues.RESULTS：（1） The rhein intervention group was significantly different from blank group（P0.05）.Rhein can improve renal functions and significantly reduce the grade of renal fibrosis and interstitial inflammation（P0.05）;（2） The levels of BMP7 and HGF were markedly elevated in renal tissues of rhein intervention group（P0.05）.However,the expression of TGF-β1 was similar between these two groups. CONCLUSION：Rhein can improve renal functions and reduce the grade of renal fibrosis and interstitial inflammation,which may associate with that rhein plays a role in anti-inflammation,inducing the expression of BMP7 and HGF.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2011年第10期1114-1120,共7页
Chinese Journal of Clinical Pharmacology and Therapeutics
