摘要
目的:应用RAPD技术鉴别通关藤及其混淆品,并分析同种不同产地通关藤基因同源性。方法:CTAB法提取7个不同产地的通关藤及其6种混淆品的DNA,以20条随机引物进行非特异扩增。结果:随机引物285(GGG AAC CCG T)可稳定扩增不同产地通关藤药材的DNA,随机引物E01(CCC AAG GTC C)可有效的鉴别通关藤及其混淆品。结论:RAPD法可准确鉴别正品通关藤及其混淆品,不同产地通关藤的基因具有同源性。
Objective：To identify Marsdeniae Tenacissimae Caulis and its adulterants by RAPD（random amplified polymorphic DNA） and analyze the gene homology of Marsdeniae Tenacissimae Caulis from various habitats. Methods：General DNA was isolated from Marsdeniae tenacissimae Caulis which were from seven various habitats and its six adulterants by CTAB,the twenty RAPD was used to identify them.Results：Random primer 285（GGG AAC CCT T）could amplify the gene of Marsdeniae tenacissimae Caulis from various habitats stablely,Marsdeniae tenacissimae Caulis and its adulterants could be identified by primer E01（CCC AAG GTC C）effectively.Conclusion：The method of RAPD can be used to identify Marsdeniae Tenacissimae Caulis and its adulterants,the gene of Marsdeniae tenacissimae Caulis from various habitats have homology.
出处
《中药材》
CAS
CSCD
北大核心
2011年第9期1355-1357,共3页
Journal of Chinese Medicinal Materials
基金
中国博士后科学基金特别资助项目(200902550)
辽宁省教育厅基金项目(2009T006)
辽宁省科技厅博士启动基金项目(20101069)
关键词
通关藤
混淆品
RAPD
鉴别
Marsdeniae Tenacissimae Caulis
Adulterants
RAPD
Identification
