摘要
目的:探讨胆道梗阻大鼠外周血中性粒细胞(polymorphonuclear neutrophil,PMN)趋化性与IL-8水平改变的意义。方法:将55只SD大鼠随机分为正常组(A组)、假手术组(B组)和胆总管结扎组(C组),B、C组术后又分为1、3、7、10、14d等5个时相。采用改良的Boyden方法检测PMN的趋化性,ELISA双抗体夹心法检测血清IL-8水平。结果:C组PMN的趋化性从术后1d(19.78±2.98)/HPF增加至14d(38.09±2.99)/HPF,高于A组(7.34±2.04)/HPF及B组相应时相;血清IL-8水平从术后1d(313.64±89.99)pg/mL逐步升至14d(547.73±81.93)pg/mL,高于A组(140.63±21.74)pg/mL及B组相应时相。并且C组PMN趋化性与IL-8水平存在显著正相关性。结论:胆道梗阻大鼠外周血PMN趋化性的增加与IL-8水平异常升高有关,两者相互影响,共同参与梗阻性黄疸的病理过程,在过度和持续炎症反应中具有重要的作用和意义。
Objective To investigate the significance of alteration of peripheral polymorphonuclear neutrophil（PMN） chemotaxis and the serum level of Interleukin-8（IL-8） in a rat model with biliary obstruction.Methods 55 SD adult rats were randomly divided into three groups：normal control group（A）,sham-operation group（B） and bile duct ligation（BDL） group（C）.Subsequently,Group B and Group C were randomly separated into subgroups of day 1,3,7,10 and 14.Blood samples were collected,serum IL-8 was detected by ELISA technique and PMN chemotaxis was evaluated with the modified Boyden method.Results Group C displayed significantly enhanced chemotaxis from day 1（19.78±2.98）/HPF to day 14（38.09±2.99）/HPF,as well as increased level of serum IL-8 from day 1（313.64±89.99）pg/ml to day 14（547.73±81.93） pg/ml,when compared to group A（7.34±2.04）/HPF,（140.63±21.74） pg/ml and corresponding phase subgroup of Group B.In addiction,there was an obviously positive correlation between PMN chemotaxis and the serum level of IL-8 in Grounp C.Conclusion The enhancement of PMN chemotaxis is intimately associated with the abnormally increased level of IL-8 in BDL rats.Both are interacted each other.Elevation of serum level of IL-8 could cause the increasing PMN chemotaxis,which might induce excessive and persistent inflammation and severe septic complications in the development of obstructive jaundice.
出处
《深圳中西医结合杂志》
2011年第5期257-260,共4页
Shenzhen Journal of Integrated Traditional Chinese and Western Medicine
基金
深圳市科技计划资助项目(200902050)
关键词
胆道梗阻
中性粒细胞
趋化性
细胞因子
IL-8
Biliary obstruction； Polymorphonuclear neutrophil； Chemotaxis； Cytokine； Interleukin-8
