摘要
目的:通过体外试验,探讨RGD偶联吉西他滨白蛋白纳米粒(RGD-BSANP-GEM)对胰腺癌细胞株BxPC-3的体外增殖的抑制作用。方法:以人胰腺癌细胞株BxPC-3为研究对象,分为5个给药组:BSANP对照组、RGD-BSANP组、BSANP-GEM组、GEM原药组、RGD-BSANP-GEM组。运用MTT法检测给药后24 h、48 h和72 h的增殖抑制率。结果:BSANP-GEM组细胞抑制率高于GEM组(P<0.05),RGD-BSANP-GEM组细胞抑制率高于BSANP-GEM组。同时RGD-BSANP-GEM组对胰腺癌细胞株的抑制率与给药浓度和作用时间呈正相关。结论:体外RGD环肽能够增加BSANP-GEM对胰腺癌细胞的增殖抑制作用。
Objective To explore the inhibitory effects of RGD-conjugated gemcitabine（GEM）-loaded albumin nanoparticles（ANP） on the proliferation of BxPC-3 cells in vitro.Methods Human pancreatic carcinoma BxPC-3 cells were cultured and then treated 5 different combinations of drugs（BSANP controls,RGD-BSANP,BSANP-GEM,GEM and RGD-BSANP-GEM） respectively.The inhibition rate of BxPC-3 cells was detected by MTT.Results The gemcitabine-loaded albumin nanoparticles produced higher inhibitory effects than gemcitabine alone.The RGD-conjugated gemcitabine-loaded albumin nanoparticles produced even higher anti-proliferation rate than those without RGD conjugation.Conclusions RGD peptides can increase the anti-proliferation rate of gemcitabine-loaded albumin nanoparticles on BxPC-3 cells in vitro.
出处
《外科理论与实践》
2011年第5期470-473,共4页
Journal of Surgery Concepts & Practice
基金
上海市经委引进技术消化吸收再创新项目(07XI-028)
