摘要
目的:建立以紫外分光光度法测定支扩养阴颗粒中二氢黄酮含量的方法。方法:以橙皮苷为对照品,四氢硼钠为还原剂,在紫外吸收光谱283nm波长处测定二氢黄酮的含量。结果:供试品与对照品有相近的特征紫外吸收光谱;二氢黄酮以橙皮苷计,进样量在100～710μg范围内与吸光度呈良好线性关系(r=0.9999),平均加样回收率为99.89%,RSD=0.66%(n=9)。结论:本方法简易、可行、精密度好,可用于支扩养阴颗粒的质量控制。
OBJECTIVE： To establish UV spectrophotometry for the content determination of flavonone in Zhikuo yangyin granules. METHODS： Flavonone was identified by UV spectrophotometry at the detection wavelength of 283 nm with hesperidin as control and sodium tetrahydroborate as reductant. RESULTS： The preparations showed characteristic spectrum similar to reference substances. The amount of flavonone in the granules was measured with hesperidin. A good linear range was shown within the rang of 100 μg to 710 μg（r=-0.999 9）. The average recovery was 99.89% （RSD=0.66% ,n=9）. CONCLUSION： The method is simple, feasible and precise. It can be used for the quality control of Zhikuo yangyin granules.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第43期4083-4085,共3页
China Pharmacy
基金
上海市肺科医院院级课题(fk1014)
