摘要
目的 建立慢性氟中毒后认知功能障碍大鼠模型,探讨慢性氟中毒所致认知功能障碍的发生机制.方法 用含不同浓度氟化钠（0、50、100、150 mg/L）的蒸馏水喂养Wister大鼠6个月,建立慢性氟中毒大鼠模型;采用Y型迷宫和旷场分析实验检测模型大鼠的认知行为功能改变;H-E染色观察大鼠海马形态的变化.结果 氟中毒组大鼠学习记忆成绩低于正常对照组,中、高氟中毒组表现更为明显（P＜0.05或P＜0.01）;与正常对照组相比,高氟中毒组的海马区细胞形态出现了明显的改变.结论 大鼠慢性氟中毒所导致的认知功能障碍与高氟地区人群认知功能障碍有相似之处,可作为研究慢性氟中毒认知障碍的动物模型.
Objective To establish the rat model of cognitive dysfunction induced by chronic fluorosis and to investigate the underlying mechanism. Methods Animal model of chronic fluorosis was established by feeding Wistar rats on distillated water containing different concentrations of sodium fluoride （0, 50, 100 and 150 mg/L） for six months; Y- maze and open field test were used to evaluate the changes in cognitive ethology of rats ; the morphological changes of rat hippocampus were observed using H - E staining. Results The learning and memory abilities were lower in chronic fluorosis groups, particularly in medium and high fluorosis groups （P 〈0.05 or P 〈0.01 ） than in the normal group; Compared with the normal group, marked morphological changes were observed in the hippocampal cells in high fluorosis group. Conclusion The rat model has a strong resemblance in cognitive dysfunction caused by chronic fluorosis to that in population of high fluorosis areas, which is available to serve as an animal model to study the cognitive impairment caused by chronic fluorosis.
出处
《徐州医学院学报》
CAS
2011年第5期319-322,共4页
Acta Academiae Medicinae Xuzhou
基金
基金项目：徐州医学院2008年研究生创新课题
关键词
慢性氟中毒
认知障碍
动物模型
chronic fluorosis
cognitive dysfunction
animal model
