摘要
目的:观察竹节参总皂苷(TSPJ)对脑缺血再灌大鼠神经细胞凋亡和早期快速反应基因c-fos,c-jun表达的影响。方法:60只SD大鼠随机分为假手术组、模型组、尼莫地平组、TSPJ组(200,100,50 mg.kg-1)。线栓法制备大鼠局灶性脑缺血-再灌注损伤模型;HE染色检测脑组织病理变化;TUNEL法检测神经细胞凋亡;免疫组化方法检测c-fos,c-jun的表达。结果:脑缺血再灌注损伤后,模型组大鼠脑组织病理损伤明显,TUNEL细胞较假手术组显著增多(P<0.01),c-fos,c-jun表达较假手术组明显增强(P<0.01);TSPJ可明显改善模型大鼠脑组织病理形态,TSPJ(200,100 mg.kg-1)组与模型组相比,TUNEL阳性细胞数明显减少(P<0.01或P<0.05),c-fos,c-jun阳性表达减少(P<0.01或P<0.05)。结论:对脑缺血损伤具有保护作用,其作用可能是通过下调c-fos,c-jun蛋白表达,从而干预脑缺血后的神经细胞凋亡。
Objective：To investigate the effects of total saponins of Panax japonicus（TSPJ） on neuronal apoptosis and expression of c-fos,c-jun proteins in rat cerebral injury induced by ischemia and reperfusion.Method： Sixty SD rats were randomly divided into 6 groups：control group,model group,nimodipine group,TSPJ 200,100,50 mg·kg-1groups.The models of cerebral ischemia-reperfusion injury was reproduced by middle cerebral artery occlusion（MCAO）.HE staining was used to assay the pathological changes;TUNEL stainning was used to detect apoptotic neurons;immuno-histochemistry was used to observe the protein expression of c-fos and c-jun.Result： Compared with the control group,the neuronal apoptosis was increased and the expression of c-fos、c-junhad remarkably enhanced after cerebral ischemia reperfusion（P〈0.01）;TSPJ improved the pathological changes,TSPJ（200,100 mg·kg-1）group significantly inhibited the expression of postitve neuronal cells of TUNEL and the expression of c-fos,c-jun decreased（P〈0.01,P〈0.05）.Conclusion：TSPJ can decrease the number of apoptotic cells after cerebral ischemia and reperfusion by down-regulating the expression of c-fos and c-jun,which may be one of mechanisms of neuroprotective effects.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第21期168-172,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
北京市优秀人材资助项目(20051D0501823)
首都中医药与护理专项基金(11zyh07)
