摘要
目的探讨快速冷冻切片技术改进方法在病理诊断中的应用价值。方法对我院2010年手术切除的乳腺、甲状腺、子宫、喉、脑组织等600例新鲜组织标本,采用医用耦合剂包埋,恒温式冰冻切片机切片,苏木素加热伊红快速染色后封片观察。结果此方法制片时间约8 min,镜下组织结构和组织切面完整,细胞形态清晰,染色佳,组织无冰晶形成及碎裂。结论该方法简便快速,冷冻切片质量好,可为快速准确地进行病理诊断提供保证。
Objective To discuss the application value of improvement methods of quick frozen sections preparation in the pathological diagnosis. Methods 600 tissues of mammary glands, thyroid glan, uterus, larynx and brain selected in surgery during 2010 were observed using medical couping agent, constant temperature type frozen section machine slice, fast staining of heating haematoxylin and eosin and sealing. Results The production time was 8 min, microscopical organization structure and slice organization were complete, cells form was clear-cut and dyeing was perfect, and there was no crystallization and cleft. Conclusion This method is simple, quick frozen sections are of good quality and can provide pathological diagnosis quickly and accurately.
出处
《解放军医药杂志》
CAS
2011年第5期4-5,I0002,共3页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
关键词
冷冻切片
组织包埋
染色与标记
Frozen section
Tissue embedding
Staining and labeling
