摘要
目的探讨微波辐照对大鼠外周血淋巴细胞亚群的影响及其可能机制。方法健康雄性清洁级Wistar大鼠,经0、5、10、30和60mW/cm2不同功率密度微波辐照后1、7、14、28d,用流式细胞仪检测外周血CD3+、CD4+、CD8+T细胞、CD4+/CD8+比值和CD45RA+B细胞,观察其变化情况。结果辐照后7d,10～30mW/cm2组和14d的5～30mW/cm2组CD3+T细胞均较对照组降低,差异有统计学意义(P<0.05)。辐照后14d,10mW/cm2组CD4+T细胞较对照组降低,差异有统计学意义(P<0.01)。辐照后1～14d各辐照组CD8+T细胞均较对照组降低,其中30mW/cm2组的降低具有统计学意义(P<0.05)。辐照后1d的5mW/cm2组CD4+/CD8+比值较对照组升高,辐照后14d的5～30mW/cm2组CD4+/CD8+比值较对照组降低,差异有统计学意义(P<0.05)。与T细胞变化趋势相反,微波辐照后各组CD45RA+B细胞均较对照组升高,其中辐照后1d30mW/cm2组和辐照后14d30～60mW/cm2组CD45RA+B细胞较对照组升高,差异有统计学意义(P<0.05或P<0.01),且在辐照后14d呈较好的量效关系。结论一定剂量的微波辐照能诱发大鼠外周血淋巴细胞亚群比例改变并导致免疫功能受损。
Objective To investigate the effects and mechanisms of microwave radiation on peripheral lymphocyte subpopulations in Wistar rats.Methods A total of 100 Wistar rats（180-220g） were exposed to microwave with different average power densities of 5,10,30 and 60 mW/cm2,and sham exposure of 0mW/cm2 was performed in a control group at the same time.At day 1,7,14 and 28 after microwave irradiation,the changes in peripheral CD3＋,CD4＋,CD8＋ T cells,ratio of CD4＋/CD8＋ and CD45RA＋ B lymphocyte in rats were analyzed by flow cytometry（FCM）.Results The CD3＋ T cells decreased significantly in 10-30mW/cm2 groups at day 7 and in 5-30 mW/cm2 groups at day 14 after radiation as compared with control group（P0.05）,and CD4＋ T cells decreased significantly in 10mW/cm2 group at day 14 after radiation as compared with control group（P0.01）.From day 1 to day 14 after radiation,CD8＋ T cells showed a reduction in number in all irradiated groups when compared with the control,but statistical significance was only found in the 30mW/cm2 group（P0.05）.The CD4＋/CD8＋ ratio increased in 5mW/cm2 group on day 1,while decreased significantly in 5-30mW/cm2 groups on day 14 after radiation as compared with control group（P0.05）.After microwave exposure,however,CD45RA＋ B cells in 30mW/cm2 group at day 1 and in 30-60mW/cm2 groups at day 14 after radiation increased significantly in a dose-dependent manner.Conclusion A definite dosage of microwave radiation,ranging from 5-60mW/cm2,may induce changes in subpopulations of peripheral lymphocytes and cause acute immune function impairment in rats.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2011年第10期1045-1047,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家科技重大专项基金
国家自然科学基金(39000386)
毫米波国家重点实验室开放课题(K200909)
军队医药卫生"十一五"基金(09MA026)
