摘要
目的探讨抗辐灵对微波辐射后大鼠免疫损伤的影响。方法采用30 mW/cm^2的微波辐射120只二级雄性Wistar大鼠,各组大鼠于辐射后当天开始,每天1次,连续14 d。药物组给予抗辐灵,药物浓度分别为0.75 g/kg/d、1.5 g/kg/d、3 g/kg/d,安多霖药物组给予安多霖,药物浓度为1.5 g/kg/d,正常对照组和辐射对照组均给予等比体积的蒸馏水。各组大鼠分别于辐射后7 d(给药7 d)、14 d(停药后6 h)和21 d(停药后7 d)抽取外周血,应用全自动血细胞分析仪检测白细胞(white blood cell,WBC)计数、淋巴细胞计数及比例(lymphocyte count,LYM)、红细胞(red blood cell,RBC)计数、血红蛋白(hemoglobin,Hb)和血小板总数(platelet,PLT)等血液细胞学指标,应用流式细胞仪检测CD_4^+/CD_8^+比值。结果 30 mW/cm^2辐射后14 d(停药后6 h),辐射对照组与正常对照组比,大鼠外周血WBC有降低趋势;与辐射对照组比,抗辐灵药物组大鼠外周血WBC明显升高(P<0.05);辐射后7 d,辐射对照组和安多霖药物组大鼠外周血RBC较正常对照组明显升高(P<0.05),抗辐灵药物组与正常对照组相比无显著性差异,与辐射对照组和安多霖药物组相比显著减少(P<0.05或0.01);辐射后14 d(停药后6 h),与正常对照组相比,辐射对照组和安多霖药物组LYM明显降低(P<0.05);与辐射对照组相比,抗辐灵药物组LYM明显升高(P<0.05);与安多霖药物组相比,1.5 g/kg/d和3 g/kg/d抗辐灵药物组LYM明显升高(P<0.05);辐射后各时间点,各组之间HGB和PLT无显著性差异。辐射后7 d(给药7 d),辐射对照组CD_4^+/CD_8^+比值明显降低(P<0.05)。结论抗辐灵对30 mW/cm^2微波辐射引起的大鼠免疫损伤具有保护作用,效果优于安多霖,1.5 g/kg/d为最佳剂量。
Objective To study the Effect of Kang Fu Ling（KFL） on immune injury induced by microwave exposure in rats. Materials and methods 120 male Wistar rats were irradiated with 30 mW/cm^2 and orally administrated with KFL（0. 75,1. 5,3 g/kg/d）,ADL（1. 5 g/kg/d） or water after radiation,once/d,successive administration for 14 d. The counts of leucocytes,lymphocytes,red blood cells,hemoglobin and platelets,ratio of CD4＋/CD8＋were detected with cell count and flow cytometry at 7th day,14 th day and 2th day after the microwave exposure. Results 14 days after the exposure,the counts of leucocytes in radiation group decreased trend compared with control group and KFL group（P 0. 05）was significant increase than radiation group; 7 days after the exposure,the counts of red blood cells in radiation group and ADL group than control group increased significantly（P 0. 05）,KFL group was no significant difference compared with control group whereas significantly reduced compared with radiation group and ADL group; 14 days after the exposure,the counts of lymphocytes in radiation group and ADL group（P 0. 05） were decreased obviously than control group; KFL group（P 0. 05） was significantly increased than radiation group; KFL group（1. 5,3 g/kg/d）（P 0. 05） was significant increase than ADL group; The counts of hemoglobin and platelets were not significantly different among the 6 groups after the exposure. 7 days after the exposure,the ratio of CD_4~＋/CD_8~＋in radiation group（P 0. 05） was significantly lower than control group. Conclusions Treatment with 1. 5 g/kg/d KFL is approved to protect against immune injury caused by 30 mW/cm^2 microwave exposure in rats and the effect is better than that of ADL.
出处
《中国体视学与图像分析》
2017年第1期45-50,共6页
Chinese Journal of Stereology and Image Analysis
基金
国家重大专项资助(2011ZXJ09303-06)
关键词
抗辐灵
微波
大鼠
免疫损伤
保护作用
定量分析
Kang Fu Ling
microwave
rat
immune injury
protective effect
quantitative analysis
