摘要
目的:分析高尔基体α-甘露糖苷酶Ⅱ(GMⅡ)在不同分化人胃癌细胞系和胃癌组织中的差异表达,以探讨GMⅡ在胃癌发生发展中的作用。方法:收集38例胃腺癌及30例癌旁正常胃黏膜组织,体外培养3株不同分化胃癌细胞系(高分化MKN-28、中分化SGC-7901、低分化BGC-823)和永生化正常胃黏膜上皮细胞系(GES-1),分别应用RT-PCR、免疫组化和Western Blotting检测GMⅡmRNA和蛋白的表达。结果:GMⅡ阳性表达定位于胞浆,其在正常胃粘膜组织及高、中、低分化胃癌组织中的阳性表达率分别为53%(16/30)、63%(5/8)、83%(15/18)和100%(12/12),各组间差异显著(P<0.05)。细胞爬片显示GMⅡ在正常胃黏膜上皮细胞系和高、中、低分化胃癌细胞系中的表达逐渐增强。与正常胃黏膜上皮细胞系GES-1相比,3种胃癌细胞系MKN-28、SGC-7901、BGC-823中GMⅡmRNA及蛋白表达水平显著增加(P<0.05),且GMⅡ在低分化胃癌细胞系BGC-823中表达最高,而在高分化胃癌细胞系MKN-28中表达最低。与正常胃黏膜组织相比,高、中、低分化胃癌组织中GMⅡmRNA及蛋白表达水平亦显著增加(P<0.05),且GMⅡ表达水平在高分化胃癌组织中最低,而在低分化胃癌组织中最高。结论:GMⅡ参与了胃癌的发生和发展,其表达水平与胃癌低分化程度更密切相关。
AIM：To explore the role of Golgi mannosidase Ⅱ（GMⅡ） in the development of gastric carcinoma by analysis of the relationship between differential expression of GMⅡ and differentiation of gastric carcinoma cell lines and tissues.METHODS： Thirty cases of human normal gastric tissues and 38 cases of gastric adenocarcinoma tissues were selected.Three different differentiated gastric carcinoma cell lines（MKN-28,SGC-7901 and BGC-823） and a normal gastric epithelial cell line GES-1 were cultured in vitro.The mRNA levels of GMⅡ were detected by RT-PCR,and the protein expression was detected by immunohistochemistry and Western blotting.RESULTS： GMⅡ was mainly distributed in cytoplasm.The positive rates of GMⅡ in 30 cases of human normal gastric tissues,8 cases of well-differentiated,18 cases of moderately-differentiated and 12 cases of poorly-differentiated gastric cancer tissues were 53%（16/30）,63%（5/8）,83%（15/18） and 100%（12/12）,respectively.The expression of GMⅡ was gradually increased in normal gastric epithelial cell line and in well,moderately and poorly-differentiated gastric cancer cell lines by cell-attached coverslip.Compared with normal gastric epithelial cell line,3 gastric carcinoma cell lines showed the higher expression of GMⅡ at mRNA and protein levels（P〈0.05）.Furthermore,GMⅡ expression in poorly-differentiated gastric carcinoma cell line BGC823 was the highest,and the lowest expression of GMⅡ was the well-differentiated cell line MKN-28.Compared with normal gastric epithelial tissues,gastric carcinoma tissues showed the higher expression of GMⅡ at mRNA and protein levels（P〈0.05）,and the highest was the poorly-differentiated carcinoma tissues.The expression of GMⅡ at mRNA and protein levels in normal gastric tissues was the lowest.CONCLUSION： GMⅡ is involved in the development and progression of gastric cancer.The expression of GMⅡ is highly related to the poorly-differentiated gastric cancer.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2011年第10期1890-1895,共6页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30672431)
教育部博士点基金资助项目(No.20060631006)
重庆市自然科学基金资助项目(No.2010BB5361)
