摘要
目的:探讨肝肠-钙黏连蛋白(liver-intestine cadherin,CDH17)过表达对胃癌BGC-823细胞增殖、侵袭及迁移的影响。方法:采用脂质体转染法建立CDH17基因过表达的BGC-823细胞株;分别采用实时荧光定量-PCR(real-time fluorogenic quantitative-PCR,RFQ-PCR)和蛋白质印迹法检测转染重组表达载体pcDNA3.1(-)/CDH17后,BGC-823细胞中CDH17mRNA和蛋白的表达水平。MTT法检测CDH17基因过表达对BGC-823细胞增殖的影响;体外侵袭实验及划痕实验检测CDH17基因过表达对BGC-823细胞迁移及侵袭能力的改变。结果:RFQ-PCR及蛋白质印迹法检测结果显示,转染重组表达载体pcDNA3.1(-)/CDH17的BGC-823细胞中CDH17mRNA及蛋白均过表达;CDH17过表达能明显提高BGC-823细胞的增殖、侵袭及迁移能力(P<0.05)。结论:CDH17基因在BGC-823细胞中过表达能提高胃腺癌的增殖、侵袭及迁移能力,并促进胃腺癌的发生及发展。
Objective： To investigate the effects of CDH17 （liver-intestine cadherin） over-expression on cell proliferation, invasion and migration of human gastric carcinoma BGC-823 cells in vitro. Methods： The BGC-823 cells over-expressing CDH17 were constructed using Lipofectamine. The expression levels of CDH17 mRNA and protein in BGC-823 cells transfected with pcDNA3.1（-）/CDH17 were detected by RFQ- PCR （real-time fluorogenic quantitative-PCR） and Western blotting, respectively. The proliferative activity of BGC-823 cells was detected by MTT method. The migration and invasive capabilities of BGC-823 cells were detected by scratch-wound migration assay and Transwell invasion assay, respectively. Results： The results of RFQ-PCR and Western blotting revealed that the expression levels of CDH17 mRNA and protein in BGC-823 cells transfected with pcDNA3.1（-）/CDH17 were over-expressed. The proliferation of BGC-823 cells was increased significantly due to CDH17 over-expression （P 〈 0.05）. The invasive and migration capabilities of BGC-823 cells transfected with pcDNA3.1（-）/CDH17 were also significantly strengthened （P 〈 0.05）. Conclusion： Overexpression of CDH17 may play an important role in the proliferation, invasion and migration of human gastric carcinoma BGC-823 cells and it can promote the oncogenesis and progression of human gastric carcinoma.
出处
《肿瘤》
CAS
CSCD
北大核心
2012年第11期880-885,共6页
Tumor
关键词
胃肿瘤
细胞黏附分子
细胞增殖
细胞浸润
CDH17基因
Stomach neoplasms
Cell adhesion molecules
Cell proliferation
Neoplasm invasiveness
CDH17 gene
