摘要
目的:观察血清胸腺因子9肽对人结肠癌HT-29移植瘤的抑制作用。方法:裸鼠皮下接种人结肠癌HT-29细胞,瘤块生长至体积约100mm^3后,分为血清胸腺因子9肽高、中、低剂量组(分别为1.25、0.625、0312mg/kg)、环磷酰胺阳性对照组(30mg/kg)和生理盐水空白对照组,每组10只,血清胸腺因子9肽各剂量组和空白对照组裸鼠每天皮下给受试物1次,连续28d。阳性对照组腹腔注射给药,每周2次,连续4周。每周称量体质量、测量瘤块长径和短径2次。于末次给药后24h处死动物,称取体质量、测量瘤块长径和短径,计算体积后,剥离肿瘤称瘤质量,计算相对肿瘤增殖率和抑瘤率。实验重复3次。结果:血清胸腺因子9肽高、中、低剂量组相对肿瘤体积与空白对照组比较均显著减小(P<0.01或P<0.05),其相对肿瘤增殖率分别为50%±20%、62%±20%和77%±35%;血清胸腺因子9肽高、中、低剂量组瘤质量与空白对照组比较均明显减轻(P均<0.01),抑瘤率分别为45%±3%、35%±1%、27%±3%;给药前后血清胸腺因子9肽各剂量组裸鼠体质量与空白对照组比较变化不显著(P>0.05)。结论:血清胸腺因子9肽对人结肠癌HT-29移植瘤具有明显抑制作用。
OBJECTIVE：To observe the anticancer effect of the serum thymic factor（FTS） 9 peptide on human colon cancer HT-29 transplantation model in nude mice.METHODS：Nude mice were inoculated subcutaneously with human colon cancer HT-29 cells,grouping was performed when tumor reach a volume of approximately 100 mm^3.Tumorbearing nude mice were divided into 5 groups：FTS 9 peptide high,medium and low dose group（1.25,0.625,0.312 mg/kg,respectively）,positive control（cyclophosphamide,30 mg/kg） and blank control（saline）,10 mice in each group. Mice were treated subcutaneously once a day for 28 days.Body weight,length and width of tumor were measured twice a week.Mice were sacrificed 24 h after the last treatment,body weight,length,width,volume and weight of tumor were measured,the relative tumor proliferation and inhibition rates were calculated.Experiment was repeated 3 times. RESULTS：Relative tumor volumes of FTS 9 peptide groups were significantly reduced compared with blank control （P0.01 or P0.05）,relative tumor proliferation rates of FTS 9 peptide high,medium and low dose groups were 50%±20%,62%±20%and 77%±35%,respectively.Tumor weights of FTS 9 peptide groups were significantly reduced compared with blank control（P0.01）,inhibition rate of FTS 9 peptide high,medium and low doses group were 45%±3%,35%±1%and 27%±3%,respectively.Body weights of nude mice in each group were not significantly different （compared with blank control,P0.05）.CONCLUSION：The results showed that the FTS 9 peptide had an obvious inhibitory effect on transplanted tumor of human colon cancer HT-29.
出处
《癌变．畸变．突变》
CAS
CSCD
2011年第5期374-376,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
