摘要
微生物基因组测序和高通量分析方法获得了大量的数据和信息,利用这些信息研究代谢网络成为当前的一个新热点。文章在比较和分析重构代谢网络不同方法的基础上,利用蜡状芽胞杆菌群中已测序的9株蜡状芽胞杆菌、6株炭疽芽胞杆菌、6株苏云金芽胞杆菌基因组,对它们的碳水化合物代谢途径、氨基酸代谢途径和能量代谢途径进行比较与分析,找出它们的共性和特性。这3种菌都存在必需的糖酵解、三羧酸循环、丙氨酸代谢、组氨酸代谢及能量代谢等途径;同时它们还存在特殊的代谢途径,蜡状芽胞杆菌对单糖的利用率较高;炭疽芽胞杆菌的氨基酸降解和转运途径较丰富;苏云金芽胞杆菌中存在催化谷氨酸转化的代谢旁路等。代谢途径的分析为深入研究它们的食物毒素、炭疽毒素和杀虫毒素提供了新思路。
A large number of data and information was obtained from genome sequencing and high-throughput genomic studies,use of the information to study metabolic networks become a new hotspot in biological research.This article com-pared different methods to reconstruct metabolic networks and analyzed the advantages and disadvantages of each methods,and then introduced some researches about carbohydrate metabolism pathways,amino acid metabolic pathways,and energy metabolism pathways of 9 strains of Bacillus cereus,6 strains of B.anthracis,,6 strain of B.thuringiensis,and finds out their similarities and characteristics.These three strains have some necessary metabolic pathways,such as glycolysis,tri-carboxylic acid cycle,alanine metabolism,histidine metabolism,and energy metabolism,but they may have some specific pathways.B cereus has higher efficiency in utilizing monosaccharide,B.anthracis is rich in degradation and transport pathways of amino acids.A glutamate metabolic bypass way exists in B.thuringiensis.Analysis of metabolic pathways provides a new way to study and use food toxin,anthrax toxin,and insecticidal toxin of these strains in future.
出处
《遗传》
CAS
CSCD
北大核心
2011年第10期1057-1066,共10页
Hereditas（Beijing)
基金
国家自然科学基金重点项目(编号:30930004)资助
关键词
蜡状芽胞杆菌群
代谢网络
代谢途径分析
Bacillus cereus group
metabolic networks
analysis of metabolic pathways
