摘要
目的:探讨多孔钛(titanium,Ti)支架材料修复节段性骨缺损的疗效。方法:新西兰大白兔30只,切除兔双前肢桡骨中下段1 cm连同骨膜的骨质造成骨缺损,选择左侧前肢为实验侧,右侧前肢为对照侧。实验侧缺损区植入多孔钛,对照侧缺损区植入多孔羟基磷灰石(hydroxyapatite,HA)。于术后8、12、24周行组织学和组织形态学观察,进行图像分析,测其新生骨面积,术后12、24周时行力学测试。结果:组织学显示:两组孔内长入大量新生骨质,随着术后时间的延长,在12、24周时实验侧新骨细胞长入量和骨重建效果与对照组接近。两组种植体均与骨组织实现骨紧密结合,未见纤维间隔。组织形态学结果也显示,试验组和对照组的新生骨面积(S bone)占植入区总面积(S total)的百分比(S)在新生骨质长入量上差异无统计学意义(P>0.05)。而12、24周时3点弯曲实验显示,12周时最大承载负荷(N)分别为107.34±27.44(Ti组)和93.42±21.18(HA组);24周时最大承载负荷分别为118.56±24.65(Ti组)和102.15±23.37(HA组)。实验组力学性能优于对照组,但差异无统计学意义(P=0.102)。结论:多孔钛支架材料可以促进新骨的形成并有利于长段骨缺损的修复,其促进新骨生成能力和修复节段性骨缺损效果与多孔羟基磷灰石相似。
Objective：To investigate the therapeutic effects of porous titanium（Ti） on the recovery of rabbit radial bone defect.Methods： Bone defects were artificially made in 30 New Zealand rabbits by resecting the 1 cm substantial osseo with periosteum of both radii.The left anterior limbs were implanted with porous titanium,while the right anterior limbs with porous hydroxyapatite（HA）.The rabbits were sacrificed at three time points.Both the radii healing statuses were observed by histology and histomorphometry analyses by means of computer graphic processing at the end of 8,12 and 24 weeks,and biomechanical analyses at the end of 12 and 24 weeks.Results： The histology examination showed that mass newly formed bone had grown into most pores of both the specimens.Along with prolongation of times after operation,the ingrowths of bone cells and effects of bone remodeling in the research side were nearly the same as those in the control side at the end of 12 and 24 weeks.The interface between the new bone and implants showed tight contact in both the groups without an obvious fibrous tissue.The results of histomorphometrical analyses showed that a statistically significant difference was not obtained for % bone area（bone area/ gross implant） between both the groups（P0.05）.However,the results of maximum failure load for the Ti group and the HA group were（107.34±27.44） N and（93.42±21.18） N at the end of 12 weeks,（118.56±24.65） N and（102.15±23.37） N at the end of 24 weeks,respectively.Biomechanical properties of the Ti group was stronger than that of the HA group,however,a statistically significant difference was not obtained between both the groups（P=0.102）.Conclusion： Porous titanium scaffold can promote the formation of new bone,which contributes to the healing of long tubular bone defect.The porous titanium can enhance the bone repairing effect on segmental bone defect nearly the same as porous hydroxyapatite.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2011年第5期724-729,共6页
Journal of Peking University:Health Sciences
基金
"十一五"国家科技支撑计划重点项目(2007BAI04B06)
国家重点基础研究发展计划(973计划
G2005cb623901)资助项目~~
关键词
骨代用品
桡骨
骨折
不愈合
生物力学
兔
Bone substitutes
Radius
Fractures
ununited
Biomechanics
Rabbits
