摘要
目的进一步观察吡喹酮对曼氏裂头蚴感染小鼠的疗效。方法将72只小鼠分为8组(每组9只),每只小鼠经口感染5条裂头蚴,感染后1周1-3组分别应用2 000、2 800、3 600mg/kg吡喹酮治疗1个疗程(每日3次,疗程3d)后1周剖杀,4-6组治疗2个疗程后1周剖杀,7、8组为对照组。另选40只小鼠分为4组(每组10只),每只经口感染5条裂头蚴,感染后14周1～3组应用2 000mg/kg吡喹酮治疗后1、3、5周剖杀;4组为对照组。各组小鼠剖杀后收集裂头蚴数并计算各组的平均检出虫数和减虫率,光镜下观察虫体形态变化。结果小鼠感染裂头蚴后1周,应用2 000、2 800、3 600mg/kg吡喹酮治疗1个疗程后的减虫率分别为70.6%、77.3%及84%(P>0.05),治疗2个疗程后的减虫率分别为57.1%、54.6%及54.6%(P>0.05)。小鼠感染裂头蚴后14周,应用2 000mg/kg吡喹酮治疗后1、3及5周的减虫率分别只有28%、20%及20%(P>0.05);虽然治疗后裂头蚴虫体有断裂现象,体壁上出现突起、溃烂及溶解等,但虫体头部无明显破坏。结论增加剂量与疗程不能提高吡喹酮对裂头蚴感染小鼠的疗效;吡喹酮对裂头蚴病的治疗效果可能与感染后的治疗时间有关。
In order to further study the efficacy of praziquantel on mice infected with Sparganum mansoni,72 mice were divided into 8 groups（9 mice in each group）,and each mice was orally infected with 5 sparana.At one week after infection,1-3 groups were treated with praziquantel（2 000,2 800,and 3 600mg/kg in three doses per day for 3 days）,and sacrificed at 1 week after 1 course;4-6 groups were treated with the second course of praziquantel as the same dosage,and sacrificed at 1 week after 2 courses;7 and 8 groups were used as control.Additional 40 mice were divided into 4 groups（10 micein each group）,and each mice was orally infected with 5 sparana.At 14 weeks after infection,1-3 groups were treated with praziquantel（2 000mg/kg）,and sacrificed at 1,3,and 5 weeks after treatment.After the infected mice were sacrificed,the number of worms recovered was observed and worm reduction rate was determined,and the morphological changes of sparana post treatment were observed under microscope.When treating with one course of 2 000,2 800,and 3 600mg/kg of praziquantel at 1 week post infection,the worm reduction rate in three groups of the infected mice was 70.6%,77.3% and 84%（P0.05）.After treatment with two course of praziquantel,the worm reduction rate was 57.1%,54.6% and 54.6%（P0.05）.When the infected mice were treated with 2 800mg/kg of praziquantel at 14 weeks post infection,the worm reduction rate in the infected mice was only 28%,20% and 20% at 1,3 and 5 weeks after treatment（P0.05）.After treatment with praziquantel,the spargana was ruptured,and the protuberance and dissolution of cuticles were observed,but the destruction of the anterior end of spargana were not found.The results showed that the increase of dosage and course of treatment did not improve the therapeutic effect of praziquantel on mice infected with sparganum,and the efficacy of praziquantel for sparganosis might be related to time of therapy after infection.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2011年第10期921-924,共4页
Chinese Journal of Zoonoses
基金
河南省医学科技攻关(201003006)资助项目
关键词
裂头蚴病
治疗
吡喹酮
小鼠
sparganosis
treatment
praziquantel
mice
