摘要
采用滤纸片法测定蟾皮不同溶剂提取物抑菌性能,平皿二倍稀释法测定最小抑菌浓度(MIC),并进一步考察其抑菌活性部位的量效关系与耐热耐压稳定性。结果表明,蟾皮水提物、30%乙醇提取物、水提醇沉物对供试菌没有抑菌作用,而60%乙醇提取物与90%乙醇提取物对供试菌有不同程度的抑制作用,并且90%乙醇提取物的抑菌效果强于60%乙醇提取物;90%乙醇提取物对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌、黑曲霉的最小抑菌浓度(MIC)分别为1 000,500,500,500mg/mL;抑菌活性与总生物碱的提取量存在一定的量效关系;抑菌活性部位具有一定的耐热耐压稳定性。
Paper disc method was used to observe the antibacterial ac-tivity of extracts from the skin of bufo bufo with different solvents on tested strains.The minimal inhibitory concentration（MIC）was test-ed by plate two-fold dilution method.And the dose-effect relation-ship and the heat-pressure stability of the antimicrobial active frac-tion of the skin of bufo bufo were also studied.The results showed that the water extracts,30%alcohol extracts and ethanol sediments from the skin of bufo bufo had no bacteriostasis activities on the tested strains.The 60%ethanol extracts and the 90%ethanol extracts had bacteriostasis activities on the tested strains inordinately and the 90%ethanol extracts was better than the 60%ethanol extracts.The MIC of 90%ethanol extracts to Eschrichia coli,Staphyl-ococcus aureus,Bacillus subtilis and Aspergillus niger were 1 000 mg/mL,500mg/mL,500 mg/mL,500 mg/mL,respectively.There were dose-effects relationship between the inhibitory effect and total alkaloid,and the antimicrobial active fraction had heat-pressure stability.
出处
《食品与机械》
CSCD
北大核心
2011年第5期120-122,共3页
Food and Machinery
基金
湖南省教育厅科学研究项目资助(编号:11C1321)
中南林业科技大学青年科学研究基金资助(编号:2009015B)
湖南省高校"中药新药创制与资源综合持续利用"科技创新团队资助(编号:湘教通[2010]212号)
关键词
蟾皮
抑菌
稳定性
the skin of bufo bufo
bacteriostasis
stability
