摘要
背景:血管平滑肌细胞增殖是心血管系统组织变化的基础。目的:观察不同浓度香烟提取物对大鼠主动脉平滑肌细胞结缔组织生长因子表达的影响。方法:取第3代大鼠主动脉平滑肌细胞,分别用体积分数2.5%,5%,10%,20%的香烟提取物进行干预。结果与结论:实时定量RT-PCR结果显示,体积分数2.5%,5%,10%,20%的香烟提取物作用12h均可促进大鼠主动脉平滑肌细胞结缔组织生长因子mRNA的表达,以体积分数5%香烟提取物的作用最明显,且其刺激大鼠主动脉平滑肌细胞结缔组织生长因子mRNA的表达在24h内呈时间依赖性。提示香烟提取物可上调大鼠主动脉平滑肌细胞结缔组织生长因子mRNA的表达,导致大鼠主动脉平滑肌细胞的增殖。
BACKGROUND：Vascular smooth muscle cell proliferation is the basis for changes of cardiovascular tissue. OBJECTIVE：To investigate the effects of different concentrations of cigarette smoke extract on the expression of connective tissue growth factor in rat aortic smooth muscle cells. METHODS：Passage 3 rat aortic smooth muscle cells were interfered with 2.5%, 5%, 10% and 20% cigarette smoke extract. RESULTS AND CONCLUSION： Real-time quantitative PCR results showed that treatment with 2.5%, 5%, 10% and 20% cigarette smoke extract for 12 hours can increase mRNA expression of connective tissue growth factor in rat aortic smooth muscle cells, in particular 5% cigarette smoke extract, and that mRNA expression of connective tissue growth factor in rat aortic smooth muscle cells was dose-dependent within 24 hours. Results demonstrated that cigarette smoke extract can upregulate the mRNA expression of connective tissue growth factor in rat aortic smooth muscle cells, leading to the proliferation of rat aortic smooth muscle cells.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第37期6901-6904,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金资助项目(30871074)
课题名称:Egr-1与骨桥蛋白相互调控机制及在血管再狭窄中的作用~~
