摘要
目的:分析桥本甲状腺炎(HT)外周血中CD4+CD28-及CD8+CD28-T细胞表达变化,探讨其在HT发病中的作用。方法:采用流式细胞仪测定50例初诊HT患者及30例正常对照者外周血CD4+及CD8+T细胞表面CD28分子的表达。结果:HT组与对照组比较,CD4+比例增高而CD8+比例降低,CD4+/CD8+比值增加;CD4+CD28+和CD8+CD28+在外周血表达下降,而CD4+CD28-和CD8+CD28-则表达增加;血清中IFN-γ及hs-CRP水平明显高于对照组;以上差别均有统计学意义(P<0.05)。且HT患者CD4+CD28-和CD8+CD28-表达比例分别与血清中IFN-γ和hs-CRP水平呈正相关(r=0.383,P=0.006;r=0.309,P=0.029)。结论:HT存在免疫调节功能异常,CD4+和CD8+T淋巴细胞表面的CD28的表达均明显减少,CD4+CD28-T细胞增加可能诱导了疾病的发生,CD8+CD28-T细胞增加可能参与HT的发病过程,导致患者出现慢性炎症反应。
Objective：To study the expressions of CD4^＋CD28^-and CD8＋CD28^-T cells in peripheral blood of patients with Hashimoto′s Thyroiditis（HT）.Methods： CD28 on CD4^＋ and CD8＋ T cells were detected in 50 cases with HT and 30 cases with healthy conditions by flow cytometric analysis.Results： CD4^＋ T cells,CD4^＋/CD8＋ increased,CD8＋ T cells decreased in HT group when compared with healthy group.In peripheral blood,the expressions of CD4^＋CD28＋ and CD8＋CD28＋ decreased,CD4^＋CD28^-and CD8＋CD28^-increased.IFN-γ and hs-CRP in serum were much higher than those in healthy group.These differences which described above had statistical significances（P〈0.05）.There were positive correlations between CD4^＋CD28^-T cells and IFN-γ（r=0.383,P=0.006）,CD8＋CD28^-T cells and hs-CRP（r=0.309,P=0.029）.Conclusion： There are abnormal functions in immune regulation proved by the reductions of CD28 on CD4^＋ and CD8＋ T cells.It is supposed that CD4^＋CD28^-T cells may induce the occurence of HT,while CD8＋CD28^-T cells may be involved in its pathogenic process.
出处
《中国卫生检验杂志》
CAS
2011年第10期2470-2471,共2页
Chinese Journal of Health Laboratory Technology
